Cárdaba B, De Pablo R, Vilches C, Martín E, Geller-Bernstein C, De Andrés B, Zaharan Y, Del Pozo V, Gallardo S, De Arruda Chaves E, Waisel Y, Palomino P, Kreisler M, Lahoz C
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Clin Exp Allergy. 1996 Mar;26(3):316-22.
We have recently described the association between the IgE antibody response to Ole e I (the major antigen from olive tree pollen) and the DR7-DQ2 haplotype in a Spanish population.
Due to the linkage disequilibrium between DR7 and DQ2, and thus the difficult distinction between the role of these two antigens in the T-cell activation response, we decided to solve this question by two approaches: 1. The study of another ethnic group, individuals of Arabic origin, with a presumably distinct disequilibrium linkage between DR and DQ antigens. Genomic DNA typing was performed in 46 subjects (allergic and non-allergic) by Restriction Fragment Length Polymorphism (RFLP) and results showed that patients with specific IgE antibodies alpha-Ole e I, were DR7 and/or DQ2. These data show a similar restriction pattern to those previously described for Spanish patients. The phenotypic frequency of DR7 antigen is significantly greater than in the non-allergic population, with a corrected P (Pc) value of 0.03. 2. The analysis of the genetic requirements of Ole e I response, using T-cell lines specific for this antigen. This was first carried out by blocking the proliferative response of these T-cell lines with specific anti-human HLA class II antibodies and then testing the genetic restriction of this response using a panel of histocompatible and histoincompatible Antigen Presenting Cells (APCs). Both experiments corroborate the hypothesis that DR7 and DQ2 are implicated in the recognition of Ole e I.
我们最近描述了西班牙人群中针对油橄榄花粉主要抗原Ole e I的IgE抗体反应与DR7-DQ2单倍型之间的关联。
由于DR7和DQ2之间存在连锁不平衡，因此难以区分这两种抗原在T细胞激活反应中的作用，我们决定通过两种方法解决这个问题：1. 研究另一个种族群体，即阿拉伯裔个体，他们的DR和DQ抗原之间可能存在不同的不平衡连锁。通过限制性片段长度多态性（RFLP）对46名受试者（过敏和不过敏）进行基因组DNA分型，结果显示具有特异性IgE抗体α-Ole e I的患者为DR7和/或DQ2。这些数据显示出与先前描述的西班牙患者相似的限制性模式。DR7抗原的表型频率显著高于非过敏人群，校正P（Pc）值为0.03。2. 使用针对该抗原的T细胞系分析Ole e I反应的遗传需求。首先通过用特异性抗人HLA II类抗体阻断这些T细胞系的增殖反应，然后使用一组组织相容性和组织不相容性抗原呈递细胞（APC）测试该反应的遗传限制性。两个实验都证实了DR7和DQ2参与识别Ole e I的假设。
