Cárdaba B, Vilches C, Martín E, de Andrés B, del Pozo V, Hernández D, Gallardo S, Fernández J C, Villalba M, Rodríguez R
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Hum Immunol. 1993 Dec;38(4):293-9. doi: 10.1016/0198-8859(93)90557-h.
We have studied the relationship between HLA class II haplotypes and alleles, and the IgE antibody response to a highly purified allergen, Ole e I, in allergic patients. Ole e I, is the major antigen from the pollen of olive tree that grows mainly in the Mediterranean. Genomic DNA typing was performed in 40 unrelated patients with seasonal allergic pollenosis who had specific IgE antibodies against Ole e I, detected by double-antibody radioimmunoassay. HLA-DRB and -DQB loci were analyzed by PCR-SSO and RFLP. Phenotypic frequencies were compared with those of 179 healthy unrelated individuals. Significant increases in the phenotypic frequencies of DR7 (pf = 67.5% vs 31.3% in the control population, pc = 0.0023) and DQ2 (pf = 90.0% vs 48.0%, pc = 0.0003) were found, indicating an association between DRB10701/2, DQB10201 alleles and the IgE antibody response to Ole e I. This is the first time that the HLA-DQ gene has been associated with a positive allergic response.
我们研究了HLA II类单倍型和等位基因与过敏性患者中针对高度纯化变应原油橄榄果 Ole e I的IgE抗体反应之间的关系。Ole e I是主要生长在地中海地区的橄榄树花粉中的主要抗原。对40例患有季节性过敏性花粉症且通过双抗体放射免疫测定法检测到针对Ole e I的特异性IgE抗体的无亲缘关系患者进行了基因组DNA分型。通过PCR-SSO和RFLP分析HLA-DRB和-DQB基因座。将表型频率与179名健康无亲缘关系个体的表型频率进行比较。发现DR7（表型频率：67.5% 对比对照组的31.3%，pc = 0.0023）和DQ2（表型频率：90.0% 对比48.0%，pc = 0.0003）的表型频率显著增加，表明DRB10701/2、DQB10201等位基因与针对Ole e I的IgE抗体反应之间存在关联。这是首次发现HLA-DQ基因与阳性过敏反应相关。
