Garlipp C R, Bottini P V, Teixeira A T
Department of Clinical Pathology, State University of Campinas, Brazil.
Rev Inst Med Trop Sao Paulo. 1995 Sep-Oct;37(5):467-9. doi: 10.1590/s0036-46651995000500014.
Human infection by Cryptosporidium spp and other coccidia are due to opportunist non-host specific microorganisms. In HIV seropositive patients, the gastrointestinal symptoms accompanying such infections may be serious and prolonged and may include nausea, low-grade fever, abdominal cramps, anorexia and watery diarrhoea. We studied 188 stool samples from 111 patients (84 men and 27 women) with diarrhoea. A modified Ziehl-Nielsen technique for the detection of Cryptosporidium spp and Isospora belli was employed. The mean age of the patients was 31 years. Cryptosporidium spp was seen in 18% (n = 20) of the patients, 90% (n = 18) of whom were HIV seropositive. Isospora belli was recorded only from HIV seropositive patients (5.4% of all the patients studied and 6.5% of those who were HIV seropositive). These data confirm the good results obtained with this technique for the identification of Cryptosporidium spp and other coccidia and also reaffirm the clinical importance of correctly diagnosing the cause of diarrhoea, particularly in HIV seropositive patients.
人类感染隐孢子虫属及其他球虫是由机会性非宿主特异性微生物引起的。在HIV血清阳性患者中，此类感染伴随的胃肠道症状可能严重且持续时间长，可能包括恶心、低热、腹部绞痛、厌食和水样腹泻。我们研究了111例腹泻患者（84名男性和27名女性）的188份粪便样本。采用改良齐-尼氏技术检测隐孢子虫属和贝氏等孢球虫。患者的平均年龄为31岁。18%（n = 20）的患者检测到隐孢子虫属，其中90%（n = 18）为HIV血清阳性。仅在HIV血清阳性患者中检测到贝氏等孢球虫（占所有研究患者的5.4%，占HIV血清阳性患者的6.5%）。这些数据证实了该技术在鉴定隐孢子虫属和其他球虫方面取得的良好结果，也再次肯定了正确诊断腹泻病因的临床重要性，尤其是在HIV血清阳性患者中。
