Kajiwara I, Kawamura K, Hiratsuka Y, Takebayashi S
Second Department of Pathology, School of Medicine, Fukuoka University, Japan.
Nephron. 1996;72(4):637-43. doi: 10.1159/000188952.
The present study was designed to determine whether the administration of free radical scavengers, superoxide dismutase (SOD), catalase or dimethylsulfoxide (DMSO) is able to ameliorate ischemia/reperfusion injury in the canine kidney and also ascertain whether or not a relationship exists between oxygen free radicals and membrane-bound Na(+)-K(+)-ATPase activity. In 23 dogs, the vascular pedicle of the left kidney was clamped for 75 min at room temperature. The experimental animals received free radical scavengers for 30 min starting at 2 min prior to reperfusion. Renal tissue specimens were enzyme-histochemically examined regarding the activity of membrane-bound Na(+)-K(+)-ATPase, and a marked reduction just before reperfusion was revealed. The SOD- and the DMSO-treated groups showed a marked recovery of the membrane-bound Na(+)-K(+)-ATPase activity; however, the untreated and the catalase-treated groups still demonstrated a marked reduction 1 day after reperfusion. At the same time, widespread acute tubular necrosis in the cortex was observed in the untreated and catalase groups in comparison with the SOD and the DMSO groups. In addition, the SOD and the DMSO groups significantly preserved better renal function. Based on these findings, it was thus concluded that free radical scavengers ameliorate the recovery of depressed membrane-bound Na(+)-K(+)-ATPase activity and ischemia/reperfusion injury in the canine kidney.
本研究旨在确定给予自由基清除剂、超氧化物歧化酶（SOD）、过氧化氢酶或二甲基亚砜（DMSO）是否能够改善犬肾缺血/再灌注损伤，并确定氧自由基与膜结合的Na(+)-K(+)-ATP酶活性之间是否存在关联。在23只犬中，将左肾血管蒂在室温下夹闭75分钟。实验动物在再灌注前2分钟开始接受自由基清除剂30分钟。对肾组织标本进行酶组织化学检查，以检测膜结合的Na(+)-K(+)-ATP酶活性，结果显示再灌注前该活性显著降低。SOD和DMSO处理组的膜结合Na(+)-K(+)-ATP酶活性显著恢复；然而，未处理组和过氧化氢酶处理组在再灌注1天后仍显示显著降低。同时，与SOD组和DMSO组相比，未处理组和过氧化氢酶组的皮质出现广泛的急性肾小管坏死。此外，SOD组和DMSO组的肾功能保存得明显更好。基于这些发现，得出结论：自由基清除剂可改善犬肾中膜结合的Na(+)-K(+)-ATP酶活性降低和缺血/再灌注损伤的恢复情况。
