Yang S, Healey M C, Du C
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-5600, USA.
FEMS Immunol Med Microbiol. 1996 Feb;13(2):141-5. doi: 10.1016/0928-8244(95)00096-8.
The present study was undertaken to determine the infectivity of Cryptosporidium parvum oocysts for immunosuppressed adult C57BL/6N mice after the oocysts had been stored from 1-48 months at 4 degrees C in 2.5% potassium dichromate. All mice inoculated with oocysts 1-18 months old developed patent infections, while mice inoculated with older oocysts remained uninfected. The prepatent period was extended from 2 to 6 or 7 days as the storage time for oocysts increased. The finding that C. parvum oocysts remain infective for mice for at least 18 months offers important economic and time-saving advantages for investigators who frequently require large numbers of oocysts that must be painstakingly purified from calf manure.
本研究旨在确定微小隐孢子虫卵囊在2.5%重铬酸钾溶液中于4℃保存1至48个月后，对免疫抑制的成年C57BL/6N小鼠的感染性。所有接种1至18个月龄卵囊的小鼠均发生显性感染，而接种较老龄卵囊的小鼠未被感染。随着卵囊保存时间的增加，潜隐期从2天延长至6或7天。微小隐孢子虫卵囊对小鼠至少18个月仍具感染性这一发现，为经常需要从牛粪中费力纯化大量卵囊的研究人员提供了重要的经济和省时优势。
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