一种宿主蛋白在微小隐孢子虫感染的上皮细胞膜上的过表达及定位
Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells.
作者信息
Yang Yi-Lin, Serrano Myrna G, Sheoran Abhineet S, Manque Patricio A, Buck Gregory A, Widmer Giovanni
机构信息
Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA.
出版信息
Mol Biochem Parasitol. 2009 Nov;168(1):95-101. doi: 10.1016/j.molbiopara.2009.07.004. Epub 2009 Jul 22.
Abstract
The genus Cryptosporidium includes several species of intestinal protozoan parasites which multiply in intestinal epithelial cells. The impact of this infection on the transcriptome of cultured host cells was investigated using DNA microarray hybridizations. The expression of 14 genes found to be consistently up- or down-regulated in infected cell monolayers was validated with RT PCR. Using immunofluorescence we examined the expression of Protease Activated Receptor-2, which is encoded by one of the up-regulated genes. In infected cells this receptor localized to the host cell membrane which covers the intracellular trophozoites and meronts. This observation indicates that the composition of the host cell membrane is affected by the developing trophozoite, a phenomenon which has not been described previously.
摘要
隐孢子虫属包括几种肠道原生动物寄生虫，它们在肠道上皮细胞中繁殖。利用DNA微阵列杂交技术研究了这种感染对培养的宿主细胞转录组的影响。通过逆转录聚合酶链反应（RT PCR）验证了在感染的细胞单层中发现的14个基因的表达持续上调或下调。我们利用免疫荧光检测了蛋白酶激活受体-2的表达，该受体由上调基因之一编码。在感染的细胞中，这种受体定位于覆盖细胞内滋养体和裂殖体的宿主细胞膜上。这一观察结果表明，发育中的滋养体影响宿主细胞膜的组成，这一现象此前尚未被描述过。
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