Magnuson M L, Owens J H, Kelty C A
Treatment Technology Evaluation Branch, National Risk Management Research Laboratory, Water Supply and Water Resources Division, Office of Research and Development, United States Environmental Protection Agency, Cincinnati, Ohio 45268, USA.
Appl Environ Microbiol. 2000 Nov;66(11):4720-4. doi: 10.1128/AEM.66.11.4720-4724.2000.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawed Cryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns for C. parvum were distinguishable from those obtained for Cryptosporidium muris. One spectral marker appears specific for the genus, while others appear specific at the species level. Three different C. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)用于研究完整的和冻融的微小隐孢子虫卵囊。完整的卵囊显示出一些质谱特征。用冻融程序裂解卵囊后,获得了可重复的光谱标记模式并提高了灵敏度。微小隐孢子虫的光谱标记模式与鼠隐孢子虫的光谱标记模式不同。一个光谱标记似乎是该属特有的,而其他的则在种水平上具有特异性。研究了三批不同的微小隐孢子虫,每批都观察到了相似的光谱标记。对卵囊进行消毒可减少和/或消除光谱标记模式。