Ren D, Wu C
Department of Pathology, Tongji Medical University, Wuhan.
Zhonghua Bing Li Xue Za Zhi. 1995 Dec;24(6):375-7.
To comprehend the relationship between the distribution of perivascular neuropeptides and remodified structure of arterioles in hypertension, we applied the technique of sheeting from pia matter of spontaneously hypertensive rats (SHR) to reveal branches at all levels of the vascular network so as to make an overall and stereoscopic observation of the vasculature. In addition, immunohistochemical technique and image analyser were applied to analyse qualitatively and quantitatively neuropeptide Y(NPY)-containing innervation in the vasculature. Findings revealed that the surface density of NPY-containing innervation and integral luminous density of positive reaction particle for anti-NPY in middle cerebral artery and its branches -small arteries and arterioles of SHR were significantly increased as compared with findings in Wistar Kyoto. The results reveal that an increase in density of NPY-containing innervation of cerebral small arteries and arterioles intensifies action of nutritive adjustment of vascular smooth muscle and is clearly related with increase of contractive excitability of artery, hypertrophy of artery, smooth muscle cells and the development of hypertension.
为了了解高血压状态下血管周围神经肽分布与小动脉结构重塑之间的关系，我们采用从自发性高血压大鼠（SHR）软脑膜铺片的技术，以显示血管网络各级分支，从而对脉管系统进行全面、立体的观察。此外，应用免疫组织化学技术和图像分析仪对脉管系统中含神经肽Y（NPY）的神经支配进行定性和定量分析。结果显示，与Wistar Kyoto大鼠相比，SHR大脑中动脉及其分支——小动脉和微动脉中含NPY神经支配的表面密度以及抗NPY阳性反应颗粒的整体光密度显著增加。结果表明，脑小动脉和微动脉中含NPY神经支配密度的增加增强了血管平滑肌营养调节作用，且明显与动脉收缩兴奋性增加、动脉肥大、平滑肌细胞以及高血压的发展有关。
