Wagenmann M, Baroody F M, Desrosiers M, Hubbard W C, Ford S, Lichtenstein L M, Naclerio R M
HNO-Klinik der Medizinischen Einrichtungen, Heinrich-Heine-Universität Düsseldorf, Germany.
Clin Exp Allergy. 1996 Apr;26(4):371-8.
Multiple mediators including prostaglandin D2 and leukotriene B4 have been shown to increase in nasal secretions during the early response to nasal challenge with antigen.
Our objective was to investigate the time course of prostanoid and leukotriene B4 release into nasal secretions on both the ipsilateral and contralateral side after a unilateral nasal allergen challenge.
We performed a controlled, randomized trial. Six volunteers were challenged unilaterally with antigen or diluent in a randomized order and discs were used to collect nasal secretions from both nostrils at 2 min intervals for 20 min after the challenge. Prostanoids and leukotriene B4 (LTB4) in recovered nasal secretions were measured by combined capillary gas chromatography-negative ion chemical ionization mass spectrometry (GC/MS).
Nasal allergen challenge resulted in a significant and immediate increase in symptoms and sneezing. PGD2 was significantly elevated above diluent values (0.6 +/- 0.6 pg) 30 s after removal of the allergen disc (P < 0.05), reached its peak (423.2 +/- 182.4 pg) at 2 min and then slowly decreased. PGD2 also increased on the contralateral side after unilateral allergen challenge, reaching peak values about six times lower than on the ipsilateral side (70.8 +/- 21.7 pg at 6 min). Levels of 9a, 11b-PGF2 after antigen provocation became significantly higher than after diluent (0 +/- 0 pg) on the ipsilateral side at 2 min (17.2 +/- 5.9 pg), and reached peak levels at 4 min (25.1 +/- 8.0 pg). LTB4 also increased significantly on the side of challenge. For the other prostanoids measured (PGF2, PGF2 alpha, TxB2, 6kPGF1 alpha), no significant changes in either ipsilateral or contralateral secretions were observed after allergen challenge.
Our study described the kinetics of PGD2 and LTB4 release as well as the contralateral release of PGD2.
多项介质，包括前列腺素D2和白三烯B4，已被证明在抗原鼻激发试验的早期反应中，鼻分泌物会增加。
我们的目的是研究单侧鼻过敏原激发试验后，同侧和对侧鼻分泌物中前列腺素类和白三烯B4释放的时间进程。
我们进行了一项对照、随机试验。6名志愿者按随机顺序接受单侧抗原或稀释剂激发试验，激发试验后每隔2分钟用滤片从双侧鼻孔收集鼻分泌物，共收集20分钟。通过毛细管气相色谱-负离子化学电离质谱联用仪（GC/MS）测定回收鼻分泌物中的前列腺素类和白三烯B4（LTB4）。
鼻过敏原激发试验导致症状和打喷嚏显著且立即增加。去除过敏原滤片后30秒，PGD2显著高于稀释剂值（0.6±0.6皮克）（P<0.05），在2分钟时达到峰值（423.2±182.4皮克），然后缓慢下降。单侧过敏原激发试验后，对侧的PGD2也增加，峰值约为同侧的六分之一（6分钟时为70.8±21.7皮克）。抗原激发后，同侧9α,11β-PGF2在2分钟时显著高于稀释剂激发后（0±0皮克）（17.2±5.9皮克），并在4分钟时达到峰值水平（25.1±8.0皮克）。激发侧的LTB4也显著增加。对于所测定的其他前列腺素类（PGF2、PGF2α、TxB2、6kPGF1α），过敏原激发试验后同侧或对侧分泌物均未观察到显著变化。
我们的研究描述了PGD2和LTB4释放的动力学以及PGD2的对侧释放情况。
