（-）-司来吉兰对大鼠老年黑质的氧化损伤保护作用
Protection of the aged substantia nigra of the rat against oxidative damage by (-)-deprenyl.
作者信息
de la Cruz C P, Revilla E, Steffen V, Rodríguez-Gómez J A, Cano J, Machado A
机构信息
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Spain.
出版信息
Br J Pharmacol. 1996 Apr;117(8):1756-60. doi: 10.1111/j.1476-5381.1996.tb15350.x.
Abstract
- We have studied the effect of (-)-deprenyl on the oxidative damage that the rat substantia nigra suffers during aging. 2. (-)-Deprenyl (2 mg kg-1, three times a week) administered for two months, beginning at 22 months of age, produced a significant increase in tyrosine hydroxylase (TH) activity (2.67 +/- 0.40 and 3.64 +/- 0.38 nmol mg-1 protein h-1 in untreated aged rats and treated aged rats respectively, P < 0.05) and in TH amount (0.072 +/- 0.012 and 0.128 +/- 0.38 absorbance 405 nm in untreated aged and treated aged rats respectively, P < 0.05). 3. The proteins of aged rat substantia nigra showed a significant decrease of carbonyl groups in treated animals compared with saline-injected control rats (136.2 +/- 21.8 and 71.5 +/- 13.2 c.p.m. microgram-1 protein in untreated aged and treated aged rats respectively, P < 0.05). 4. The carbonyl groups measured in TH enzyme showed a statistically significant decrease (42.3%) after (-)-deprenyl treatment (471.4 +/- 73.0 and 271.9 +/- 50.00 c.p.m. in untreated aged and treated aged rats respectively, P < 0.001). 5. All these results suggest that oxidative damage produced during aging is prevented by (-)-deprenyl treatment and could explain the effect of this drug in Parkinson's disease (PD) and other degenerative diseases such as Alzheimer's disease.
摘要
- 我们研究了（-）-司来吉兰对大鼠黑质在衰老过程中所遭受的氧化损伤的影响。2. 从22月龄开始，每周三次给予（-）-司来吉兰（2毫克/千克），持续两个月，结果显示酪氨酸羟化酶（TH）活性显著增加（未处理的老龄大鼠和处理后的老龄大鼠分别为2.67±0.40和3.64±0.38纳摩尔/毫克蛋白·小时，P<0.05），TH含量也显著增加（未处理的老龄大鼠和处理后的老龄大鼠在405纳米处的吸光度分别为0.072±0.012和0.128±0.38，P<0.05）。3. 与注射生理盐水的对照大鼠相比，经处理的老龄大鼠黑质蛋白中的羰基基团显著减少（未处理的老龄大鼠和处理后的老龄大鼠分别为136.2±21.8和71.5±13.2计数/分钟·微克-1蛋白，P<0.05）。4. （-）-司来吉兰处理后，TH酶中测得的羰基基团有统计学意义的减少（42.3%）（未处理的老龄大鼠和处理后的老龄大鼠分别为471.4±73.0和271.9±50.00计数/分钟，P<0.001）。5. 所有这些结果表明，（-）-司来吉兰处理可预防衰老过程中产生的氧化损伤，这可能解释了该药物在帕金森病（PD）和其他退行性疾病如阿尔茨海默病中的作用。
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