Failli P, Tesco G, Ruocco C, Ginestroni A, Amaducci L, Giotti A, Sorbi S
Department of Pharmacology, University of Florence, Italy.
Neurosci Lett. 1996 Apr 26;208(3):216-8. doi: 10.1016/0304-3940(96)12586-6.
It has been proposed that the lack of intracellular calcium concentration ([Ca2+]i) increase induced by the potassium channel blocker tetraethylammonium (TEA) in skin fibroblast cell lines identifies patients with both sporadic and familial Alzheimer's disease (AD). In order to verify this hypothesis, the effect of TEA on [Ca2+]i was studied in single fura-2-loaded skin fibroblast cell lines available in the Tissue Bank of the Italian Research Council. Four out of eight familial AD patients (one patient with S182 mutation, one patient with E5-1 mutation and two patients with 717 Val-->Ile APP mutation) and two out of five sporadic AD patients showed a positive response to TEA, whereas five out of 11 control lines were unresponsive. Our data suggest that the absence of the TEA-induced increase in [Ca2+]i in skin fibroblast cell lines does not identify all AD patients.
有人提出，钾通道阻滞剂四乙铵（TEA）诱导的皮肤成纤维细胞系细胞内钙浓度（[Ca2+]i）缺乏增加可用于识别散发性和家族性阿尔茨海默病（AD）患者。为了验证这一假设，我们在意大利研究委员会组织库中提供的单个负载fura-2的皮肤成纤维细胞系中研究了TEA对[Ca2+]i的影响。8名家族性AD患者中有4名（1名携带S182突变的患者、1名携带E5-1突变的患者和2名携带717 Val→Ile APP突变的患者）以及5名散发性AD患者中有2名对TEA呈阳性反应，而11个对照细胞系中有5个无反应。我们的数据表明，皮肤成纤维细胞系中缺乏TEA诱导的[Ca2+]i增加并不能识别所有AD患者。
