Attarian H, Bensinger W I, Buckner C D, McDonald D L, Rowley S D
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
Bone Marrow Transplant. 1996 May;17(5):699-702.
We prospectively evaluated microbiologic cultures for a series of 1263 peripheral blood stem cell harvests collected from 376 sequential patients. The incidence of microbial contamination was 0.23% of all samples, or 47% of samples from patients not receiving systemic antibiotics. This incidence of positive microbial cultures is less than that for cultures of bone marrow (3.8%) after harvesting and before further processing. Of the three positive cultures, two grew coagulase-negative Staphylococcus and the third, Streptococcus viridans. Two patients were reinfused with cultured-positive PBSC components, and neither exhibited the same organism in subsequent blood cultures. Although microbial contamination may occur during peripheral blood stem cell collection and cryopreservation, this report indicates that PBSC contamination does not play a conspicuous role in the infectious complications of PBSC transplantation.
我们前瞻性地评估了从376例连续患者采集的1263份外周血干细胞采集物的微生物培养情况。微生物污染发生率在所有样本中为0.23%，在未接受全身抗生素治疗患者的样本中为47%。这种微生物培养阳性的发生率低于采集后及进一步处理前骨髓培养的发生率（3.8%）。在三份阳性培养物中，两份培养出凝固酶阴性葡萄球菌，第三份培养出草绿色链球菌。两名患者回输了培养阳性的外周血干细胞成分，且两人在随后的血培养中均未出现相同微生物。尽管在外周血干细胞采集和冷冻保存过程中可能发生微生物污染，但本报告表明外周血干细胞污染在外周血干细胞移植的感染并发症中并未起显著作用。
