Becker T W, Krieger G, Witte I
Carl-von-Ossietzky Universität Oldenburg, ICMB/FB7, F.R.G
Free Radic Res. 1996 May;24(5):325-32. doi: 10.3109/10715769609088030.
The aliphatic n-butyr-and n-valeraldehyde as well as the aromatic benz- and anisaldehyde induced DNA strand breaks in PM2 DNA in the presence of CuCl2. Neither aldehydes nor CuCl2 alone showed DNA breakage properties. The maximum of single strand breaks (SSBs) induced by the combination of CuCl2 and aldehydes was dependent on the CuCl2-concentration. The aliphatic aldehydes induced SSBs and double strand breaks (DSBs) at lower concentrations than aromatic aldehydes when optimal CuCl2 concentration were used. Catalase and neocuproine nearly completely inhibited strand break formation induced by aromatic aldehydes/CuCl2. The prevention of strand breaks induced by aliphatic aldehydes/CuCl2 was less effective. While the inhibition by neocuproine was only 25%, catalase was totally ineffective. In all aldehydes/CuCl2 mixtures the formation of Cu(I) was observed. The results point to different DNA damaging species produced during redox reactions of aromatic and aliphatic aldehydes in combination with CuCl2.
在氯化铜存在的情况下，脂肪族正丁酸醛和正戊醛以及芳香族苯甲醛和茴香醛会在PM2 DNA中诱导DNA链断裂。单独的醛类或氯化铜都不具有DNA断裂特性。氯化铜和醛类组合诱导的单链断裂（SSB）最大值取决于氯化铜浓度。当使用最佳氯化铜浓度时，脂肪族醛类比芳香族醛类在更低浓度下诱导SSB和双链断裂（DSB）。过氧化氢酶和新铜试剂几乎完全抑制了芳香族醛类/氯化铜诱导的链断裂形成。对于脂肪族醛类/氯化铜诱导的链断裂，预防效果较差。新铜试剂的抑制率仅为25%，而过氧化氢酶则完全无效。在所有醛类/氯化铜混合物中均观察到了Cu(I)的形成。结果表明，芳香族和脂肪族醛类与氯化铜发生氧化还原反应时会产生不同的DNA损伤物质。
