丙二醛/Cu(II)对PM2 DNA和人成纤维细胞的协同DNA损伤作用。

Synergistic DNA damaging effects of malondialdehyde/Cu(II) in PM2 DNA and in human fibroblasts.

作者信息

Vöhringer M L, Becker T W, Krieger G, Jacobi H, Witte I

机构信息

Carl-von-Ossietzky Universität Oldenburg, ICBM and FB7, Germany.

出版信息

Toxicol Lett. 1998 Feb;94(3):159-66. doi: 10.1016/s0378-4274(98)00002-2.

DOI:10.1016/s0378-4274(98)00002-2

PMID:9609318

Abstract

Malondialdehyde (MDA) is a product of lipid peroxidation (LPO). In combination with CuCl2 MDA induced single strand breaks in PM2 DNA whereas MDA or CuCl2 alone had no effect. Cu(II) oxidized MDA by a radical mechanism under formation of Cu(I). DNA strand break induction was inhibited by catalase (98%), neocuproine (76%) and DMSO (61%). The synergistic damaging effect of MDA and Cu(II) was also demonstrated in human fibroblasts measured by alkaline elution. The combination MDA/CuCl2 caused extensive DNA breakage while neither MDA nor CuCl2 alone induced DNA damage within the cell. Synergistic cytotoxic effects were observed 18 h after a simultaneous treatment of the cells with MDA and CuCl2 for 1 h.

摘要

丙二醛（MDA）是脂质过氧化（LPO）的产物。MDA与CuCl₂结合可诱导PM2 DNA出现单链断裂，而单独的MDA或CuCl₂则无此作用。Cu（II）通过自由基机制氧化MDA生成Cu（I）。过氧化氢酶（98%）、新铜试剂（76%）和二甲基亚砜（DMSO，61%）可抑制DNA链断裂的诱导。通过碱性洗脱法在人成纤维细胞中也证实了MDA和Cu（II）的协同损伤作用。MDA/CuCl₂组合可导致广泛的DNA断裂，而单独的MDA或CuCl₂在细胞内均未诱导DNA损伤。在用MDA和CuCl₂同时处理细胞1小时后18小时观察到协同细胞毒性作用。