Souli A, Fu-Cheng X, Chariot J, Tsocas A, Rozé C
INSERM U 410, Faculté de Médecine Xavier-Bichat, Paris.
Gastroenterol Clin Biol. 1996 Feb;20(1):8-14.
The purpose of this study was to compare the effects of peptide YY (PYY) and neuropeptide Y (NPY) on VIP- and PGE2-stimulated intestinal net water flux at three different levels of the small intestine (duodenum, jejunum, ileum), by a technique of in situ closed loops in anaesthetised rats.
VIP-stimulated net water flux was efficiently inhibited by both peptides at all three intestinal levels studied; PYY (ID50 about 30 pmol/kg.h) was 3 to 18 fold more potent than NPY. PGE2-stimulated net water flux was also efficiently inhibited in the jejunum and ileum; PYY (ID50 about 10 pmol/kg.h) was 30 to 90 fold more potent than NPY. A 30% inhibition of PGE2-stimulated net water flux could only be achieved in the duodenum with the largest dose of either peptide used in this study.
PYY and NPY display potent inhibitory effects of stimulated net water flux at the three studied levels of the small intestine, except in the PGE2-stimulated duodenum. The PYY ID50s measured suggest that PYY may have a physiological action in regulating small intestinal water flux in the rat.
本研究旨在通过麻醉大鼠原位闭环技术，比较肽YY（PYY）和神经肽Y（NPY）对小肠三个不同水平（十二指肠、空肠、回肠）血管活性肠肽（VIP）和前列腺素E2（PGE2）刺激的肠净水流的影响。
在所研究的所有三个肠水平上，两种肽均有效抑制VIP刺激的净水流；PYY（半数抑制剂量约为30 pmol/kg·h）的效力比NPY强3至18倍。PGE2刺激的净水流在空肠和回肠中也受到有效抑制；PYY（半数抑制剂量约为10 pmol/kg·h）的效力比NPY强30至90倍。在十二指肠中，仅使用本研究中所用任一肽的最大剂量才能实现对PGE2刺激的净水流30%的抑制。
PYY和NPY在小肠的三个研究水平上对刺激的净水流均显示出强大的抑制作用，但在PGE2刺激的十二指肠中除外。所测得的PYY半数抑制剂量表明，PYY可能在调节大鼠小肠水流量方面具有生理作用。
