Kobayashi H, Kamiya S, Suzuki T, Kohda K, Muramatsu S, Kurumada T, Ohta U, Miyazawa M, Kimura N, Mutoh N, Shirai T, Takagi A, Harasawa S, Tani N, Miwa T
Dept. of Internal Medicine 6, School of Medicine, Tokai University, Kanagawa, Japan.
Scand J Gastroenterol. 1996 May;31(5):428-33. doi: 10.3109/00365529609006760.
One of the features of Helicobacter pylori infection in the human stomach seems to be disordered gastric acid secretion. The effect of vacuolating toxin (VT) produced by H. pylori on gastric acid secretion was examined.
VT(+)(toxigenic) and VT(-)(nontoxigenic) strains of H. pylori were cultured in brucella broth. The culture supernatant was added to isolated parietal cells, and acid secretion and intracellular adenosine 3'5'-cyclic phosphate (cAMP) and Ca2+ levels were measured with the 14C-aminopyrine (14C-AP) method, with 125I radioimmunoassay (RIA), and with the fura-2 fluorescence method, respectively.
In the VT(+) strain a considerable inhibitory effect on 14C-AP accumulation was observed. However, the VT(-) strain had no significant effect on intracellular c-AMP and Ca2+.
The VT(+) strain of H. pylori has an inhibitory effect on gastric acid secretion, whereas the VT(-) strain does not. This inhibitory effect was not associated with the response of second messengers. It is speculated that VT produced by H. pylori has a direct action on H(+)-K+ adenosine triphosphatase in parietal cells.
人类胃部幽门螺杆菌感染的特征之一似乎是胃酸分泌紊乱。研究了幽门螺杆菌产生的空泡毒素（VT）对胃酸分泌的影响。
将幽门螺杆菌的VT（+）（产毒素）和VT（-）（不产毒素）菌株在布鲁氏菌肉汤中培养。将培养上清液加入分离的壁细胞中，分别用14C-氨基比林（14C-AP）法、125I放射免疫测定法（RIA）和fura-2荧光法测量酸分泌以及细胞内腺苷3',5'-环磷酸（cAMP）和Ca2+水平。
在VT（+）菌株中观察到对14C-AP积累有相当大的抑制作用。然而，VT（-）菌株对细胞内c-AMP和Ca2+没有显著影响。
幽门螺杆菌的VT（+）菌株对胃酸分泌有抑制作用，而VT（-）菌株则没有。这种抑制作用与第二信使的反应无关。推测幽门螺杆菌产生的VT对壁细胞中的H（+）-K+三磷酸腺苷酶有直接作用。
