从嗜佩蓝藻中分离和鉴定限制性内切酶PpeI
Isolation and characterization of the restriction endonuclease PpeI from Phormidium persicinum.
作者信息
Piechula S, Piosik J, Bielawski K, Podhajska A J
机构信息
Department of Microbiology, University of Gdańsk, Poland.
出版信息
Mol Biotechnol. 1996 Apr;5(2):97-9. doi: 10.1007/BF02789059.
PMID:8734423
Abstract
PpeI is a type II restriction endonuclease isolated from cyanobacterial strain Phormidium persicinum. The endonuclease PpeI, an isoschizomer of ApaI, recognizes the hexanucleotide sequence (5'-GGGCC/C-3') and cleaves, after the second C, producing four nucleotide 3'-cohesive ends.
摘要
PpeI是一种从蓝藻菌株嗜鱼席藻中分离出来的II型限制性内切酶。内切酶PpeI是ApaI的同裂酶，识别六核苷酸序列（5'-GGGCC/C-3'），并在第二个C之后切割，产生四个核苷酸的3'粘性末端。
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