66千道尔顿神经丝蛋白（NF-66）：序列分析与进化

The 66-kDa neurofilament protein (NF-66): sequence analysis and evolution.

作者信息

Chan S O, Chiu F C

机构信息

Saul Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

出版信息

Neurochem Res. 1996 Apr;21(4):449-55. doi: 10.1007/BF02527709.

DOI:10.1007/BF02527709

PMID:8734438

Abstract

A 2.5 kb cDNA clone encoding the mouse 66 kd neurofilament protein (NF-66) was isolated and sequenced. The deduced protein sequence contains 501 amino acid residues. Comparison of the mouse, rat and human NF-66 indicated > 90% homology in protein sequence and 85% homology in coding nucleotide sequence. A high degree of homology was observed between NF-66 and other intermediate filament proteins especially in the alpha-helical domain. Zooblot analyses suggested that the putative ancestral gene for vimentin and NF-66 was detectable in the avian. By comparison, the ancestral sequence for GFAP appeared after that for vimentin.

摘要

分离并测序了一个编码小鼠66kd神经丝蛋白（NF - 66）的2.5kb cDNA克隆。推导的蛋白质序列包含501个氨基酸残基。小鼠、大鼠和人类NF - 66的蛋白质序列比较表明，其蛋白质序列同源性>90%，编码核苷酸序列同源性为85%。在NF - 66与其他中间丝蛋白之间观察到高度同源性，尤其是在α - 螺旋结构域。动物杂交分析表明，在鸟类中可检测到波形蛋白和NF - 66的假定祖先基因。相比之下，GFAP的祖先序列出现在波形蛋白之后。