Fifis T, Costopoulos C, Vaughan J A
Commonwealth Scientific and Industrial Research Organization, Division of Animal Health, Parkville, Vic, Australia.
Vet Microbiol. 1996 Apr;49(3-4):219-33. doi: 10.1016/0378-1135(95)00195-6.
Phospholipase B (PLB) activity was present in Fusobacterium necrophorum cultures and it correlated closely with virulence and co-purified with the hemolysin/leucocidin activities. All three activities were associated with a large molecule or molecular complex (6 x 10(2)-2 x 10(3) kDa) exhibiting varying degrees of aggregation. These were present mainly in the culture medium and to a lesser extent in cell sonicates. The PLB and toxin activities were sensitive to heat, dissociating agents, proteolytic enzymes, prolonged purification regimes, freeze-drying and repeated freeze-thawing. The toxin(s) were stable over a broad range of pH, did not require divalent ions or reducing agents and could be kept for several months as an ammonium sulfate precipitate at 4 degrees C, or stored as a concentrated liquid in the presence of proteolytic inhibitors at - 20 degrees C.
坏死梭杆菌培养物中存在磷脂酶B（PLB）活性，它与毒力密切相关，并与溶血素/杀白细胞素活性共纯化。所有这三种活性都与一种大分子或分子复合物（6×10² - 2×10³ kDa）相关，该复合物表现出不同程度的聚集。它们主要存在于培养基中，在细胞超声裂解物中的含量较少。PLB和毒素活性对热、解离剂、蛋白水解酶、长时间的纯化过程、冷冻干燥以及反复冻融敏感。毒素在很宽的pH范围内稳定，不需要二价离子或还原剂，并且可以作为硫酸铵沉淀在4℃下保存几个月，或者在存在蛋白水解抑制剂的情况下作为浓缩液在-20℃下储存。
