坏死梭杆菌培养上清液因存在磷脂酶A和溶血磷脂酶而具有溶血活性。

Hemolytic activity of Fusobacterium necrophorum culture supernatants due to presence of phospholipase A and lysophospholipase.

作者信息

Abe P M, Kendall C J, Stauffer L R, Holland J W

出版信息

Am J Vet Res. 1979 Jan;40(1):92-6.

Abstract

Culture supernatants of Fusobacterium necrophorum demonstrated hemolytic activity. The hemolysin(s), which was partially purified by ammonium sulfate precipitation, was temperature-dependent and heat labile. The spectrum of hemolytic activity against various erythrocytes included rabbit, human, and dog erythrocytes. Goats, sheep, and bovine erythrocytes showed only trace hemolysis. According to results of thin-layer chromatography, the hemolysin hydrolyzed rabbit erythrocyte phosphatidyl choline, phosphatidyl ethanolamine, lysophosphatidyl choline, and bovine phosphatidyl choline. Hydrolysis of egg yolk phosphatidyl choline, bovine phosphatidyl ethanolamine, cholesterol, 1,2-dipalmitin, 1,3-dipalmitin, sphingomyelin, or triolein was not detected by thin layer chromatography. A more sensitive procedure utilizing gas-liquid chromatography revealed that, of the substrates tested, the following were bein hydrolyzed: bovine and egg yolk phosphatidyl choline, lysophosphatidyl choline, alpha-palmito-beta-eleoyl-L-alpha lecithin and alpha-oleoyl-betal-palmitoyl-L-alpha lecithin. Substrates which were weakly hydrolyzed were bovine phosphatidyl ethanolamine, DL-alpha-hosphatidyl ethanolamine dipalmitoyl, 1,2-dipalmitin, 1,3-dipalmitin, and triolein.

摘要

坏死梭杆菌的培养上清液表现出溶血活性。通过硫酸铵沉淀部分纯化的溶血素具有温度依赖性且对热不稳定。针对各种红细胞的溶血活性谱包括兔、人及犬的红细胞。山羊、绵羊和牛的红细胞仅显示微量溶血。根据薄层色谱结果，溶血素可水解兔红细胞磷脂酰胆碱、磷脂酰乙醇胺、溶血磷脂酰胆碱及牛磷脂酰胆碱。薄层色谱未检测到蛋黄磷脂酰胆碱、牛磷脂酰乙醇胺、胆固醇、1,2 - 二棕榈酸甘油酯、1,3 - 二棕榈酸甘油酯、鞘磷脂或三油精的水解。一种利用气液色谱的更灵敏方法显示，在所测试的底物中，以下底物正在被水解：牛和蛋黄磷脂酰胆碱、溶血磷脂酰胆碱、α - 棕榈酰 - β - 油酰 - L - α - 卵磷脂及α - 油酰 - β - 棕榈酰 - L - α - 卵磷脂。水解较弱的底物为牛磷脂酰乙醇胺、二棕榈酰 - DL - α - 磷脂酰乙醇胺、1,2 - 二棕榈酸甘油酯、1,3 - 二棕榈酸甘油酯及三油精。