Calzada-Wack J C, Frankenberger M, Ziegler-Heitbrock H W
Institute for Immunology, University of Munich, Germany.
J Inflamm. 1996;46(2):78-85.
Cells of the monocyte/macrophage lineage are heterogenous in that some types express the CD16 molecule (Fc receptor for IgG, type III), while others are negative. We now show that culture of human blood monocytes with interleukin-10 (IL-10) will induce high levels of cell surface CD16 within 18 hr, and this goes along with increased transcript levels. After prolonged culture for 36 hr, control cultures also become CD16 positive and this induction can be prevented by anti-IL-10, but not by anti-tumor necrosis factor (TNF) antibody or isotype control. In vitro culture of blood monocytes results in a spontaneous decrease of the myelomonocytic stem cell antigen CD33, suggesting that the cells undergo maturation. IL-10 treatment will accelerate this process and result in a further reduction of cell surface CD33. These data indicate that IL-10 promotes monocyte maturation and directs these cells to develop into CD16 positive macrophages.
单核细胞/巨噬细胞谱系的细胞具有异质性，因为某些类型表达CD16分子（IgG的Fc受体，III型），而其他类型则为阴性。我们现在表明，用人白细胞介素-10（IL-10）培养人血单核细胞会在18小时内诱导细胞表面CD16的高水平表达，这与转录水平的增加同时发生。经过36小时的长时间培养后，对照培养物也变为CD16阳性，并且这种诱导可以被抗IL-10抗体阻止，但不能被抗肿瘤坏死因子（TNF）抗体或同型对照阻止。血单核细胞的体外培养导致骨髓单核细胞干细胞抗原CD33的自发减少，表明细胞经历成熟。IL-10处理将加速这一过程并导致细胞表面CD33的进一步减少。这些数据表明IL-10促进单核细胞成熟并引导这些细胞发育成CD16阳性巨噬细胞。
