Bianchi D W, Klinger K W, Vadnais T J, Demaria M A, Shuber A P, Skoletsky J, Midura P, Diriso M, Pelletier C, Genova M, Erikson M S, Williams J M
New England Medical Center, Boston, Massachusetts, USA.
Prenat Diagn. 1996 Apr;16(4):289-98. doi: 10.1002/(SICI)1097-0223(199604)16:4<289::AID-PD843>3.0.CO;2-T.
Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescence-activated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we describe our system using a "model' male fetal cell mixed into female peripheral blood mononuclear cells. Fetal cell yields and purities were assayed by a quantitative polymerase chain reaction (qPCR) using chromosomes Y- and 7-specific sequences. Fetal cell recovery was investigated by selection of CD71+ cells or depletion of CD45+ cells. Our data demonstrated variation in fetal cell recovery for all methods tested, although CD71+ selection by FACS gave the best and most consistent results.
荧光激活细胞分选法（FACS）、免疫磁珠法和磁激活细胞分选法（MACS）。迄今为止，尚无研究直接比较使用这些方法各自回收胎儿细胞的情况。在此，我们描述了我们的系统，该系统使用混入女性外周血单个核细胞中的“模型”男性胎儿细胞。通过使用Y染色体和7号染色体特异性序列的定量聚合酶链反应（qPCR）来测定胎儿细胞产量和纯度。通过选择CD71+细胞或去除CD45+细胞来研究胎儿细胞回收情况。我们的数据表明，尽管通过FACS选择CD71+细胞可得到最佳且最一致的结果，但所有测试方法的胎儿细胞回收情况均存在差异。
