Cho K, McFarlane I D
Department of Biological Sciences, University of Hull, UK.
Neurosci Lett. 1996 May 3;209(1):53-6. doi: 10.1016/0304-3940(96)12597-0.
The anthozoan neuropeptide Antho-RWamide I (< Glu-Ser-Leu-Arg-Trp-NH2) excites contraction of endodermal muscles in sea anemones. Single electrode voltage clamp recordings from semi-intact preparations of endodermal myoepithelial cells reveal that Antho-RWamide I increases an inward Ca2+ current. Evidence for the involvement of a Ca2+ current in contraction was supported by the observation that Cd2+ abolished spontaneous contractions and reduced inward current. Contractions and inward currents induced by Antho-RWamide I were not, however, completely abolished in the presence of Cd2+. We conclude that Antho-RWamide, a putative neurotransmitter at sea anemone smooth muscle, acts by opening, either directly or indirectly, Ca2+ channels in the muscle membrane.
珊瑚虫神经肽Antho-RWamide I（<Glu-Ser-Leu-Arg-Trp-NH2）可刺激海葵内胚层肌肉收缩。对来自内胚层肌上皮细胞半完整制剂的单电极电压钳记录显示，Antho-RWamide I可增加内向Ca2+电流。Cd2+消除自发收缩并减少内向电流这一观察结果支持了Ca2+电流参与收缩的证据。然而，在Cd2+存在的情况下，Antho-RWamide I诱导的收缩和内向电流并未完全消除。我们得出结论，Antho-RWamide是海葵平滑肌中一种假定的神经递质，其作用是直接或间接打开肌肉膜中的Ca2+通道。
