布宜诺斯艾利斯的耐多药结核病暴发。分离株的DNA指纹分析。
Multidrug resistant tuberculosis outbreak in Buenos Aires. DNA fingerprinting analysis of isolates.
作者信息
Morcillo N, Alito A, Romano M I, Cataldi A, Dolmann A, Reniero A, de Kantor I N
机构信息
Hospital Dr. A. Centrángolo, Vicente López, Buenos Aires.
出版信息
Medicina (B Aires). 1996;56(1):45-7.
In order to determine the possible relationship among HIV patients coinfected with multidrug resistant tuberculosis strains who had been receiving clinical assistance in our Hospital, clinical and epidemiological information from 28 patients was collected. DNA fingerprinting by restriction fragment length polymorphism (RFLP) pattern was performed on the mycobacterial isolates from these patients, using the restriction enzyme Pvull and IS 6110 as genetic marker. A unique RFLP pattern was found in 10 isolates from 10 different patients who had a disease caused by a single strain. Our findings confirm RFLP as a reliable and useful tool to analyze TB transmission.
为了确定在我院接受临床治疗的合并感染多重耐药结核菌株的HIV患者之间可能存在的关系，我们收集了28例患者的临床和流行病学信息。使用限制性内切酶PvuII和IS 6110作为遗传标记，对这些患者的分枝杆菌分离株进行了限制性片段长度多态性（RFLP）模式的DNA指纹分析。在10例由单一菌株引起疾病的不同患者的10株分离株中发现了独特的RFLP模式。我们的研究结果证实RFLP是分析结核病传播的可靠且有用的工具。
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