Rangel E F, Lainson R, Souza A A, Ready P, Azevedo A C
Departamento de Entomologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 1996 Jan-Feb;91(1):43-50. doi: 10.1590/s0074-02761996000100007.
Phylogenetic analysis of morphometric and biological characters indicated that there are two distinct forms of Lutzomyia whitmani in Brazil: one is present both north and south of the River Amazonas in the State of Pará while the other occurs in northeast Brazil, in the State of Ceará, and further south, including the type locality in State of Bahia. The Amazonian form is reportedly neither strongly anthropophilic nor synanthropic, and it is the vector of Leishmania shawi; whereas the southern form is often collected peridomestically, while biting man, and has been found infected with Le.(V.) braziliensis. The ratio of the length of the genital filaments to that the genital pump was found to be consistently smaller in males of the Amazonian populations. A middle repetitive DNA element was isolated by differentially screening a genomic library made using Amazonian material, and the sequence was diagnostic for this form of Lu. whitmani (being absent or occurring in low copy number in the southern form). The total evidence suggests there are at least two, geographically-isolated forms of Lu. whitmani, which may represent different cryptic species.
形态测量和生物学特征的系统发育分析表明，巴西存在两种不同形态的惠特曼白蛉：一种存在于帕拉州亚马逊河以北和以南地区，另一种出现在巴西东北部的塞阿拉州以及更南部地区，包括巴伊亚州的模式产地。据报道，亚马逊形态的白蛉既不是强烈嗜人血的，也不是共栖的，它是肖维利什曼原虫的传播媒介；而南部形态的白蛉常在住宅周围采集到，会叮咬人类，并且已发现其感染了巴西利什曼原虫（Viannia亚属）。在亚马逊种群的雄性白蛉中，发现生殖丝长度与生殖泵长度的比值始终较小。通过差异筛选使用亚马逊材料构建的基因组文库，分离出一个中度重复DNA元件，该序列可诊断这种形态的惠特曼白蛉（在南部形态中不存在或拷贝数低）。总体证据表明，至少存在两种地理隔离的惠特曼白蛉形态，它们可能代表不同的隐存种。
