Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil.
Hospital Municipal de Tomé Açu, Tomé-Açu 68680-000, PA, Brazil.
Int J Environ Res Public Health. 2022 May 23;19(10):6346. doi: 10.3390/ijerph19106346.
The state of Pará has recorded seven species that cause tegumentary leishmaniasis (TL). species induce distinct immunological responses from the host and exhibit resistance to Glucantime, the first-line drug treatment for TL in Brazil.
Identify the etiology of TL in an Amazonian city in the state of Pará.
Eleven patients with TL were recruited and nasal swabs, lesion swabs, and skin fragments samples were collected. In the control group (n = 6), only the nasal swabs were collected. Polymerase Chain Reaction (PCR) amplification of the gene region was performed using the extracted DNA from the samples, from which nine patients with TL and five in the control group were positive. Products were sequenced, mounted in CAP3 software, aligned using MAFFT v.7.221, edited in Geneious software v.8.1.7, and compared and aligned with sequences available in GenBank using the BLAST tool.
For patients with TL, six molecular diagnosis at the species level ( () (n = 5/9), () (n = 1/9)) and three at the genus level ( sp. (n = 3/9)) were obtained. In the control group, four individuals were infected with sp. (n = 4/5) and (n = 1/5).
This is the first report of infection in the mucosal secretion of a healthy person in Brazil. Moreover, genetic variants were identified in the haplotypes of in the gene sequence
帕拉州已记录了七种引起皮肤利什曼病（TL）的物种。这些物种会引起宿主产生不同的免疫反应，并对 Glucantime 产生耐药性，Glucantime 是巴西治疗 TL 的一线药物。
确定帕拉州亚马逊城市 TL 的病因。
招募了 11 名 TL 患者，并采集了鼻拭子、病变拭子和皮肤碎片样本。在对照组（n=6）中，仅采集鼻拭子。使用从样本中提取的 DNA 对基因区域进行聚合酶链反应（PCR）扩增，其中 9 名 TL 患者和 5 名对照组患者的基因呈阳性。对产物进行测序，在 CAP3 软件中进行组装，使用 MAFFT v.7.221 进行对齐，在 Geneious 软件 v.8.1.7 中进行编辑，并使用 BLAST 工具与 GenBank 中可用的序列进行比较和对齐。
对于 TL 患者，在物种水平上进行了六次分子诊断（()（n=5/9），()（n=1/9））和三次在属水平上（sp.（n=3/9））。在对照组中，有 4 人感染了 sp.（n=4/5）和（n=1/5）。
这是巴西首例在健康人黏膜分泌物中感染的报告。此外，还在基因序列中鉴定出了 的单倍型的遗传变异。
