对在巴西帕拉伊巴州若昂佩索阿分离出的耐甲氧西林金黄色葡萄球菌菌株的克隆多样性分析。

Analysis of the clonal diversity of Staphylococcus aureus methicillin-resistant strains isolated at João Pessoa, state of Paraíba. Brazil.

作者信息

Santos Filho L, Silva Sader H, Bortolotto V I, Gontijo FIlho P P, Pignatari A C

机构信息

Departamento de Ciências Farmacêuticas, UFPb, Cidade Universitária, João Pessoa, PB, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 1996 Jan-Feb;91(1):101-5. doi: 10.1590/s0074-02761996000100018.

DOI:10.1590/s0074-02761996000100018

PMID:8734958

Abstract

To investigate the clonal diversity of Staphylococcus aureus strains isolated at João Pessoa, State of Paraíba, Brazil, digested genomic DNA were studied by pulsed-field gel electrophoresis (PFGE) in nine methicillin-resistant strains (MRSA) and three methicillin-sensitive strains (MSSA), selected among 67 isolates based on their antimicrobial susceptibility and epidemiology. The isolates were obtained between April and November 1992 from the Hospital of the Federal University of Paraíba, located in João Pessoa. Two MRAS isolates from the Oswaldo Cruz Hospital, São Paulo, Brazil, including an epidemic strain previously detected from different hospitals at the country were used as control. Five different patterns, were demonstrated by MRSA isolated in João Pessoa and these patterns were described in several epidemiologically unrelated hospitals in São Paulo. Our results suggest the interstate dissemination of a MRSA clone in João Pessoa which is similar to that described in other cities of Brazil.

摘要

为研究在巴西帕拉伊巴州若昂佩索阿分离出的金黄色葡萄球菌菌株的克隆多样性，对基于抗菌药敏性和流行病学从67株分离菌中挑选出的9株耐甲氧西林菌株（MRSA）和3株甲氧西林敏感菌株（MSSA）的基因组DNA消化产物进行了脉冲场凝胶电泳（PFGE）分析。这些分离菌于1992年4月至11月取自位于若昂佩索阿的帕拉伊巴联邦大学医院。来自巴西圣保罗奥斯瓦尔多·克鲁兹医院的2株MRSA分离菌，包括1株先前在该国不同医院检测到的流行菌株用作对照。若昂佩索阿分离出的MRSA显示出5种不同的图谱，这些图谱在圣保罗几家流行病学上无关联的医院中也有描述。我们的结果表明，在若昂佩索阿存在一种MRSA克隆的州际传播，这与巴西其他城市所描述的情况相似。