20世纪60年代和80年代在中国分离出的A群脑膜炎奈瑟菌III亚组菌株中的porA等位基因是相同的。
The porA alleles are identical in subgroup III serogroup A Neisseria meningitidis strains isolated in China in the 1960s and 1980s.
作者信息
Malorny B, Maiden M C, Achtman M
机构信息
Max-Planck Institut für molekulare Genetik, Berlin, Germany.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1548-50. doi: 10.1128/jcm.34.6.1548-1550.1996.
Abstract
The porA genes from serogroup A, subgroup III strains isolated in the People's Republic of China in 1966 and in 1984 and 1985 were amplified by PCR at an annealing temperature of 75 degrees C. The DNA sequences (5 strains) and the restriction patterns generated by MspI (14 strains) were identical, unlike the results reported by Peixuan et al. (Z. Peixuan, H. Xujing, and X. Li, J. Clin. Microbiol. 33:458-462, 1995). Furthermore, PCR products which were amplified at an annealing temperature of 60 degrees C, as described by Peixuan et al., were heterogeneous in our study.
摘要
对1966年以及1984年和1985年在中国分离出的A血清群、III亚群菌株的porA基因，在75℃退火温度下通过聚合酶链反应（PCR）进行扩增。DNA序列（5株）以及由MspI产生的限制性酶切图谱（14株）是相同的，这与Peixuan等人（Z. Peixuan、H. Xujing和X. Li，《临床微生物学杂志》33:458 - 462，1995年）报道的结果不同。此外，按照Peixuan等人所述在60℃退火温度下扩增的PCR产物，在我们的研究中是异质的。
相似文献
1
The porA alleles are identical in subgroup III serogroup A Neisseria meningitidis strains isolated in China in the 1960s and 1980s.20世纪60年代和80年代在中国分离出的A群脑膜炎奈瑟菌III亚组菌株中的porA等位基因是相同的。
J Clin Microbiol. 1996 Jun;34(6):1548-50. doi: 10.1128/jcm.34.6.1548-1550.1996.
2
Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China.通过分析编码1类外膜蛋白的基因中的限制性片段长度多态性对脑膜炎奈瑟菌进行分型：在中国过去40年中的疫情评估中的应用
J Clin Microbiol. 1995 Feb;33(2):458-62. doi: 10.1128/jcm.33.2.458-462.1995.
3
The porA gene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation.A群脑膜炎奈瑟菌中的porA基因：进化稳定性与遗传变异机制
Mol Microbiol. 1994 Apr;12(2):253-65. doi: 10.1111/j.1365-2958.1994.tb01014.x.
4
High prevalence of Neisseria meningitidis hypervirulent lineages and emergence of W135:P1.5,2:ST-11 clone in Southern Brazil.巴西南部脑膜炎奈瑟菌高毒力谱系的高流行率及W135:P1.5,2:ST-11克隆株的出现。
J Infect. 2008 Oct;57(4):324-31. doi: 10.1016/j.jinf.2008.07.014. Epub 2008 Sep 23.
5
Typing of Neisseria meningitidis isolates from patients with invasive disease by molecular analysis of porin genes.通过对孔蛋白基因进行分子分析对侵袭性疾病患者的脑膜炎奈瑟菌分离株进行分型。
New Microbiol. 2001 Apr;24(2):149-55.
6
[A method for determination of Neisseria meningitidis serogroup A, B, C and W by real-time PCR].[一种通过实时聚合酶链反应测定A、B、C和W群脑膜炎奈瑟菌的方法]
Zh Mikrobiol Epidemiol Immunobiol. 2014 Nov-Dec(6):35-42.
7
Microheterogeneity of serogroup A (subgroup III) Neisseria meningitidis during an outbreak in northern Ghana.加纳北部一次疫情期间A群（III亚群）脑膜炎奈瑟菌的微观异质性
Trop Med Int Health. 2000 Apr;5(4):280-7.
8
Serotype distribution, antibiotic susceptibility, and genetic relatedness of Neisseria meningitidis strains recently isolated in Italy.意大利近期分离的脑膜炎奈瑟菌菌株的血清型分布、抗生素敏感性及基因相关性
Clin Infect Dis. 2003 Feb 15;36(4):422-8. doi: 10.1086/346154. Epub 2003 Jan 30.
9
Rapid molecular identification of Neisseria meningitidis isolates using the polymerase chain reaction followed by single-stranded conformation polymorphism analysis.采用聚合酶链反应继之以单链构象多态性分析对脑膜炎奈瑟菌分离株进行快速分子鉴定。
FEMS Immunol Med Microbiol. 2005 Aug 1;45(2):143-9. doi: 10.1016/j.femsim.2005.03.003. Epub 2005 Apr 13.
10
Typing of Neisseria meningitidis by restriction analysis of the amplified porA gene.通过对扩增的porA基因进行限制性分析对脑膜炎奈瑟菌进行分型。
Pathology. 1997 May;29(2):201-5. doi: 10.1080/00313029700169864.
引用本文的文献
1
Usefulness of pulsed-field gel electrophoresis in tracking two outbreaks of invasive meningococcal disease serogroup C in British Columbia.脉冲场凝胶电泳在追踪不列颠哥伦比亚省两起侵袭性脑膜炎奈瑟菌 C 群暴发中的作用。
Can J Infect Dis Med Microbiol. 2007 Nov;18(6):363-7. doi: 10.1155/2007/295973.
2
Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis.基于聚合酶链反应的脑膜炎奈瑟菌鉴定和基因分型的实验室间比对
J Clin Microbiol. 2005 Jan;43(1):144-9. doi: 10.1128/JCM.43.1.144-149.2005.
本文引用的文献
1
Clonal spread of serogroup A meningococci: a paradigm for the analysis of microevolution in bacteria.A群脑膜炎球菌的克隆传播：细菌微进化分析的范例
Mol Microbiol. 1994 Jan;11(1):15-22. doi: 10.1111/j.1365-2958.1994.tb00285.x.
2
The porA gene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation.A群脑膜炎奈瑟菌中的porA基因：进化稳定性与遗传变异机制
Mol Microbiol. 1994 Apr;12(2):253-65. doi: 10.1111/j.1365-2958.1994.tb01014.x.
3
Comparative characterization of the iga gene encoding IgA1 protease in Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae.脑膜炎奈瑟菌、淋病奈瑟菌和流感嗜血杆菌中编码IgA1蛋白酶的iga基因的比较特征分析
Mol Microbiol. 1995 Feb;15(3):495-506. doi: 10.1111/j.1365-2958.1995.tb02263.x.
4
Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China.通过分析编码1类外膜蛋白的基因中的限制性片段长度多态性对脑膜炎奈瑟菌进行分型：在中国过去40年中的疫情评估中的应用
J Clin Microbiol. 1995 Feb;33(2):458-62. doi: 10.1128/jcm.33.2.458-462.1995.
5
Microevolution within a clonal population of pathogenic bacteria: recombination, gene duplication and horizontal genetic exchange in the opa gene family of Neisseria meningitidis.致病细菌克隆群体内的微进化：脑膜炎奈瑟菌opa基因家族中的重组、基因复制及水平基因交换
Mol Microbiol. 1994 Apr;12(2):171-80. doi: 10.1111/j.1365-2958.1994.tb01006.x.
6
Immunogenicity and evolutionary variability of epitopes within IgA1 protease from serogroup A Neisseria meningitidis.A群脑膜炎奈瑟菌IgA1蛋白酶表位的免疫原性及进化变异性
Mol Microbiol. 1994 Jan;11(1):175-87. doi: 10.1111/j.1365-2958.1994.tb00299.x.
7
Clonal population structure of Neisseria meningitidis serogroup A isolated from epidemics and pandemics between 1915 and 1983.1915年至1983年间从流行性和大流行性疫情中分离出的A群脑膜炎奈瑟菌的克隆群体结构。
Rev Infect Dis. 1987 Jul-Aug;9(4):665-92. doi: 10.1093/clinids/9.4.665.
8
Intercontinental spread of an epidemic group A Neisseria meningitidis strain.流行性A群脑膜炎奈瑟菌菌株的洲际传播
Lancet. 1989 Jul 29;2(8657):260-3. doi: 10.1016/s0140-6736(89)90439-x.
9
Comparison of the class 1 outer membrane proteins of eight serological reference strains of Neisseria meningitidis.脑膜炎奈瑟菌八株血清学参考菌株1类外膜蛋白的比较
Mol Microbiol. 1991 Mar;5(3):727-36. doi: 10.1111/j.1365-2958.1991.tb00743.x.
10
Fast and sensitive silver staining of DNA in polyacrylamide gels.聚丙烯酰胺凝胶中DNA的快速灵敏银染法。
Anal Biochem. 1991 Jul;196(1):80-3. doi: 10.1016/0003-2697(91)90120-i.
Zotero 插件