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通过逆转录聚合酶链反应检测粪便和血清中的戊型肝炎病毒RNA。

Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR.

作者信息

Turkoglu S, Lazizi Y, Meng H, Kordosi A, Dubreuil P, Crescenzo B, Benjelloun S, Nordmann P, Pillot J

机构信息

Service de Bactériologie-Virologie, Hôpital Antoine Béclère, Paris, France.

出版信息

J Clin Microbiol. 1996 Jun;34(6):1568-71. doi: 10.1128/jcm.34.6.1568-1571.1996.

DOI:10.1128/jcm.34.6.1568-1571.1996
PMID:8735122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229066/
Abstract

Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.

摘要

通过逆转录聚合酶链反应（RT-PCR）分析了在猴子实验性戊型肝炎病毒（HEV）感染期间收集的粪便和血清，以及在流行和散发病例中从急性戊型肝炎病毒感染患者收集的粪便和血清。比较了两种RNA纯化方法。在提高戊型肝炎病毒基因组检测的敏感性和特异性方面，蛋白酶K消化和酚氯仿提取比异硫氰酸胍提取更有效。

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本文引用的文献

1
Variation in course of hepatitis E in experimentally infected cynomolgus monkeys.实验感染食蟹猴中戊型肝炎病程的变化
J Infect Dis. 1993 Jun;167(6):1302-6. doi: 10.1093/infdis/167.6.1302.
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Experimental hepatitis E: pathogenesis in cynomolgus macaques (Macaca fascicularis).
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Report of a workshop: waterborne non-A, non-B hepatitis.一次研讨会的报告：水性非甲非乙型肝炎
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.采用酸性硫氰酸胍-苯酚-氯仿萃取法一步分离RNA的方法。
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Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.
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