Lemieux A M, Coe C L, Ershler W B, Karaszewski J W
Department of Psychology, University of Wisconsin, Madison 53706, USA.
Brain Behav Immun. 1996 Mar;10(1):27-43. doi: 10.1006/brbi.1996.0003.
Four experiments were conducted in aged rhesus monkeys to investigate how physical and psychological stressors influence the lymphocyte cytolytic responses against two target cell lines. An initial analysis of the lytic activity of various cell subsets against K562 and RAJI target cell lines suggested that both CD3+CD8+ and several CD3- subsets were responsible for lysis of the K562 cells. The RAJI cell line, in contrast, was killed primarily by CD3-subsets. To explore the implications of this differential mediation of lysis, cytolytic activity was evaluated after a physical challenge (surgery), a psychological disturbance (social separation), and in vitro incubation of lymphocytes with cortisol. The minor surgical procedure-laparoscopic examination-resulted in a significant decrease in lymphocyte cytolytic responses against both target cells 1 week postsurgery. In contrast, psychological disturbance elicited by changes in social relations caused a dual response, differentially affecting the lysis of either K562 or RAJI cells, dependent upon the type of behavioral reaction. Incubation of lymphocytes with cortisol in vitro indicated that lysis of both targets could be affected by corticosteroids, but the high concentration required (10(-6) M) suggested that the in vivo inhibition of cytotoxicity may not have been mediated by adrenocortical activation. Overall, the results highlight the value of utilizing multiple target cell systems in the analysis of cytolytic activity, especially in studies using nonhuman primates.
在老年恒河猴身上进行了四项实验，以研究身体和心理应激源如何影响淋巴细胞对两种靶细胞系的细胞溶解反应。对各种细胞亚群针对K562和RAJI靶细胞系的溶解活性进行的初步分析表明，CD3 + CD8 +细胞亚群和几个CD3 -细胞亚群均参与了K562细胞的溶解过程。相比之下，RAJI细胞系主要被CD3 -细胞亚群所杀伤。为了探究这种不同的溶解介导作用的意义，在经历身体挑战（手术）、心理干扰（社会隔离）以及淋巴细胞与皮质醇进行体外孵育后，对细胞溶解活性进行了评估。小型外科手术——腹腔镜检查——导致术后1周淋巴细胞对两种靶细胞的细胞溶解反应显著降低。相反，社会关系变化引起的心理干扰引发了双重反应，根据行为反应的类型不同，对K562或RAJI细胞的溶解产生不同影响。淋巴细胞与皮质醇在体外孵育表明，两种靶细胞的溶解都可能受到皮质类固醇的影响，但所需的高浓度（10(-6) M）表明体内细胞毒性的抑制可能并非由肾上腺皮质激活介导。总体而言，这些结果凸显了在细胞溶解活性分析中使用多种靶细胞系统的价值，尤其是在使用非人灵长类动物的研究中。
