Bossinger O, Schierenberg E
Zoologisches Institut Universität Köln, Germany.
Int J Dev Biol. 1996 Feb;40(1):431-9.
As more and more cases of necessary cell-cell interactions are revealed, the classical view of mosaic development in nematodes has to be replaced by a more dynamic picture showing different types of intercellular communication. To investigate the pattern and function of communication pathways between cells, we have developed different techniques to shunt fluorescent marker dyes into embryos and hatched animals and study their distribution in vivo. During embryogenesis we find that for a long time all somatic cells form a single dye-coupling compartment while transfer into the germline is restricted already at an early stage. Considerable variations between species with respect to the size of communication channels and the time during which these are functional are observed and can be correlated to differences in the developmental program. A different kind of intracellular communication can be visualized with the help of fluorescent dyes: a transfer of yolk proteins in two phases of the life cycle, in the adult hermaphrodite from the gut into the maturing germ cells, and in the embryo from non-gut cells into the gut primordium. Cell-cell interactions in the nematode embryo can be inhibited with polysulfated hydrocarbon dyes (e.g. Trypan Blue) which bind strongly to the plasma membrane. In summary our data indicate that fluorescent marker dyes can be helpful tools to identify and understand the role of intercellular communication and transfer processes in nematode development.
随着越来越多必要的细胞间相互作用的案例被揭示，线虫中经典的镶嵌发育观点必须被一幅更具动态性的画面所取代，该画面展示了不同类型的细胞间通讯。为了研究细胞间通讯途径的模式和功能，我们开发了不同的技术，将荧光标记染料导入胚胎和孵化后的动物体内，并在体内研究它们的分布。在胚胎发生过程中，我们发现很长一段时间内所有体细胞形成一个单一的染料偶联区室，而向生殖系的转移在早期就已经受到限制。观察到不同物种在通讯通道大小以及这些通道发挥功能的时间方面存在相当大的差异，并且这些差异可以与发育程序的差异相关联。借助荧光染料可以观察到另一种细胞内通讯：在生命周期的两个阶段存在卵黄蛋白的转移，在成年雌雄同体中从肠道转移到成熟的生殖细胞中，在胚胎中从非肠道细胞转移到肠道原基中。线虫胚胎中的细胞间相互作用可以被多硫酸化碳氢化合物染料（如台盼蓝）抑制，这种染料与质膜强烈结合。总之，我们的数据表明荧光标记染料可以成为识别和理解线虫发育中细胞间通讯和转移过程作用的有用工具。
