用聚合酶链反应检测血清中卡氏肺孢子虫序列。

Detection of Pneumocystis carinii sequences in serum by polymerase chain reaction.

作者信息

Miyawaki H, Fujita J, Hojo S, Harada M, Yamaji Y, Suguri S, Takahara J

机构信息

First Department of Internal Medicine, Kagawa Medical School, Japan.

出版信息

Respir Med. 1996 Mar;90(3):153-7. doi: 10.1016/s0954-6111(96)90157-2.

DOI:10.1016/s0954-6111(96)90157-2

PMID:8736207

Abstract

The clinical significance of the detection of Pneumocystis carinii DNA was evaluated, as well as the detection of circulating P. carinii antigen from serum using previously collected samples. Fourteen serum samples from 13 patients were diagnosed positively for P. carinii DNA by polymerase chain reaction (PCR). Ten of 14 episodes (71.4%) of pulmonary complications were compatible with P. carinii pneumonia. Two patients were definitely diagnosed as having had P. carinii pneumonia at autopsy. All patients positive for circulating antigens were also positive for P. carinii DNA, suggesting that the detection of P. carinii DNA by PCR is more sensitive compared to the detection of circulating antigens by the Ouchterlony method. It is concluded that the detection of P. carinii DNA in serum by PCR provides useful information for identifying P. carinii pneumonia.

摘要

评估了卡氏肺孢子虫DNA检测的临床意义，以及使用先前采集的样本从血清中检测循环卡氏肺孢子虫抗原。通过聚合酶链反应（PCR）对13例患者的14份血清样本进行卡氏肺孢子虫DNA检测，结果呈阳性。14例肺部并发症中有10例（71.4%）符合卡氏肺孢子虫肺炎。两名患者在尸检时被明确诊断为患有卡氏肺孢子虫肺炎。所有循环抗原检测呈阳性的患者卡氏肺孢子虫DNA检测也呈阳性，这表明与用琼氏免疫扩散法检测循环抗原相比，PCR检测卡氏肺孢子虫DNA更敏感。结论是，通过PCR检测血清中的卡氏肺孢子虫DNA可为识别卡氏肺孢子虫肺炎提供有用信息。