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通过聚合酶链反应（PCR）检测血液中卡氏肺孢子虫DNA对卡氏肺孢子虫肺炎的诊断没有价值。

Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia.

作者信息

Tamburrini E, Mencarini P, Visconti E, Zolfo M, De Luca A, Siracusano A, Ortona E, Wakefield A E

机构信息

Department of Infectious Diseases, Università Cattolica del S. Cuore, Rome, Italy.

出版信息

J Clin Microbiol. 1996 Jun;34(6):1586-8. doi: 10.1128/jcm.34.6.1586-1588.1996.

DOI:10.1128/jcm.34.6.1586-1588.1996
PMID:8735128
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229072/
Abstract

A nested PCR which amplified a portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was used to detect P. carinii DNA in blood from patients with P. carinii pneumonia. P. carinii DNA was not detected in serum and was detected at low levels of blood cells.

摘要

采用巢式聚合酶链反应（PCR）扩增卡氏肺孢子虫线粒体大亚基核糖体RNA基因的一部分，以检测卡氏肺孢子虫肺炎患者血液中的卡氏肺孢子虫DNA。血清中未检测到卡氏肺孢子虫DNA，血细胞中检测到低水平的该DNA。

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Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia.通过聚合酶链反应（PCR）检测血液中卡氏肺孢子虫DNA对卡氏肺孢子虫肺炎的诊断没有价值。
J Clin Microbiol. 1996 Jun;34(6):1586-8. doi: 10.1128/jcm.34.6.1586-1588.1996.
2
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J Eukaryot Microbiol. 1999 Sep-Oct;46(5):104S-105S.
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本文引用的文献

1
Variable efficiency of three primer pairs for the diagnosis of Pneumocystis carinii pneumonia by the polymerase chain reaction.用于通过聚合酶链反应诊断卡氏肺孢子虫肺炎的三对引物的可变效率。
Mol Cell Probes. 1995 Oct;9(5):333-40. doi: 10.1016/s0890-8508(95)91636-9.
2
The use of a nested polymerase chain reaction for detecting Pneumocystis carinii from lung and blood in rat and human infection.使用巢式聚合酶链反应检测大鼠和人类感染中肺及血液中的卡氏肺孢子虫。
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3
Usefulness of PCR for detection of Pneumocystis carinii DNA.聚合酶链反应检测卡氏肺孢子虫DNA的实用性
J Clin Microbiol. 1994 Sep;32(9):2324-6. doi: 10.1128/jcm.32.9.2324-2326.1994.
4
Detection of Pneumocystis DNA in serum and circulating cells is not a good diagnostic marker for Pneumocystosis in HIV patients.检测血清和循环细胞中的肺孢子菌DNA，对HIV患者肺孢子菌病来说并非良好的诊断标志物。
J Eukaryot Microbiol. 1994 Sep-Oct;41(5):112S.
5
PCR for detection of Pneumocystis carinii in blood cells.用于检测血细胞中卡氏肺孢子虫的聚合酶链反应。
J Clin Microbiol. 1995 May;33(5):1431-2. doi: 10.1128/jcm.33.5.1431-1432.1995.
6
Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens.通过对血清标本进行聚合酶链反应诊断艾滋病患者的卡氏肺孢子虫肺炎。
J Infect Dis. 1995 Dec;172(6):1623-6. doi: 10.1093/infdis/172.6.1623.
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Detection of Pneumocystis carinii with DNA amplification.采用DNA扩增技术检测卡氏肺孢子虫。
Lancet. 1990 Aug 25;336(8713):451-3. doi: 10.1016/0140-6736(90)92008-6.
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Amplification of mitochondrial ribosomal RNA sequences from Pneumocystis carinii DNA of rat and human origin.源自大鼠和人类的卡氏肺孢子虫DNA中线粒体核糖体RNA序列的扩增。
Mol Biochem Parasitol. 1990 Nov;43(1):69-76. doi: 10.1016/0166-6851(90)90131-5.
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Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia.DNA扩增技术在肺孢子菌病中的应用：人类及实验性诱导的卡氏肺孢子菌肺炎患者血清中卡氏肺孢子菌DNA的存在情况
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