Bautísta Jiménez R D, Jiménez Abizanda A I, Jiménez Moreno F J, Arias León J J
Departamento de Química Analítica, Bromatología y Toxicología, Facultad de Química, Universidad de La Laguna, Tenerife, Spain.
Clin Chim Acta. 1996 May 30;249(1-2):21-36. doi: 10.1016/0009-8981(95)06254-8.
A partial least-squares calibration (PLS) method for the simultaneous spectrofluorimetric determination of acetylsalicylic acid, codeine and ephedrine is proposed. The use of PLS for the multicomponent spectrofluorimetric determination is demonstrated by analysing synthetic mixtures. Analyte mixtures can be resolved even in the presence of strongly overlapped fluorescence signals. The method was validated by subjecting experimental data to exhaustive statistical analysis. The proposed method was applied to the simultaneous determination of the three drugs in synthetic mixtures with a urine matrix. The concentration ranges considered are 1.56-10.92 and 25-100 micrograms/ml for the codeine-ephedrine mixture, and 0.8-3.2, 0.1-0.5 and 1.6-4.8 micrograms/ml for the acetylsalicylic acid-codeine-ephedrine mixture. The analytical results were quite good in all cases.
提出了一种用于同时荧光光谱法测定乙酰水杨酸、可待因和麻黄碱的偏最小二乘校准（PLS）方法。通过分析合成混合物证明了PLS在多组分荧光光谱测定中的应用。即使在存在强烈重叠荧光信号的情况下，分析物混合物也能得到分辨。通过对实验数据进行详尽的统计分析对该方法进行了验证。所提出的方法应用于同时测定含尿液基质的合成混合物中的三种药物。可待因 - 麻黄碱混合物的浓度范围为1.56 - 10.92微克/毫升和25 - 100微克/毫升，乙酰水杨酸 - 可待因 - 麻黄碱混合物的浓度范围为0.8 - 3.2微克/毫升、0.1 - 0.5微克/毫升和1.6 - 4.8微克/毫升。在所有情况下，分析结果都相当不错。
