Saramet A, Dănilă G, Păduraru I, Petrariu D, Olinescu R
Department of Biochemistry, University of Medicine and Pharmacy, Iassy, Romania.
Arzneimittelforschung. 1996 May;46(5):501-4.
The antioxidant potential of a new water soluble flavonic derivative, namely theophylline rutoside (TR-1722) has been tested using the chemiluminescence method. The method is based on the oxidative degradation of luminol by the hydrogen peroxide in Tris-HCl-buffer, when reactive species of oxygen are being obtained: O2-., HO., 1O2, and allows for the capability of substances to inhibit the free radical processes in this test system to be quantified and hence for their antioxidant properties in respect to a standard substance (in our case quercetin) to be compared. The results obtained reveal that TR-1722 has antioxidant action comparable to that of quercetin, the highest efficacity being registered at the concentration of 2 mumol/l, the conditions being: H2O2 16,2 mmol/l; luminol 2 mumol/l, in Tris-HCl buffer 20 mmol/l, pH 8.3. The antioxidant potential of TR-1722 is also maintained when the conditions of the system are modified, that is, the concentration of the hydrogen peroxide, the intensity of the action being dependent on the hydrogen peroxide concentration, but no direct proportionality is registered.
利用化学发光法对一种新型水溶性黄酮衍生物，即茶碱芦丁糖苷（TR - 1722）的抗氧化潜力进行了测试。该方法基于在Tris - HCl缓冲液中过氧化氢对鲁米诺的氧化降解，此时会产生活性氧物种：O2-.、HO.、1O2，并能够对物质在该测试系统中抑制自由基过程的能力进行量化，从而比较其相对于标准物质（在我们的实验中为槲皮素）的抗氧化性能。所得结果表明，TR - 1722具有与槲皮素相当的抗氧化作用，在浓度为2 μmol/l时抗氧化效果最佳，条件为：H2O2 16.2 mmol/l；鲁米诺2 μmol/l，在20 mmol/l的Tris - HCl缓冲液中，pH 8.3。当改变系统条件时，即改变过氧化氢的浓度，TR - 1722的抗氧化潜力依然存在，其作用强度取决于过氧化氢浓度，但未发现直接的比例关系。
