Teselkin Iu O, Babenkova I V, Liubitskiĭ O B, Klebanov G I, Vladimirov Iu A
Department of Biophysics, Russian State Medical University, Moscow, Russia.
Vopr Med Khim. 1998 Jan-Feb;44(1):70-6.
The method of valuation of the blood plasma antioxidant activity (AOA) by the hemoglobin-hydrogen peroxide-luminol chemiluminescence system has been proposed. The method is based on the measuring the induction time of chemiluminescence that is directly proportional to the added volume of plasma or concentration of standard antioxidant. The ascorbate was taken as a standard. Blood plasma AOA has been expressed through the concentration of equivalent ascorbate solution (ascorbate equivalent). The influence of hemolysis and storage conditions of plasma on its detected AOA was investigated. The change of human blood plasma AOA after single administration of 2 g the ascorbate and its dynamics in patients with acute pancreatitis was studied.
提出了通过血红蛋白-过氧化氢-鲁米诺化学发光系统评估血浆抗氧化活性（AOA）的方法。该方法基于测量化学发光的诱导时间，该时间与血浆添加体积或标准抗氧化剂浓度成正比。以抗坏血酸作为标准。血浆AOA通过等效抗坏血酸溶液（抗坏血酸当量）的浓度来表示。研究了溶血和血浆储存条件对其检测到的AOA的影响。研究了单次给予2 g抗坏血酸后人体血浆AOA的变化及其在急性胰腺炎患者中的动态变化。
