离散的脑区表达胰岛素反应性葡萄糖转运蛋白GLUT4。

Discrete brain areas express the insulin-responsive glucose transporter GLUT4.

作者信息

Leloup C, Arluison M, Kassis N, Lepetit N, Cartier N, Ferré P, Pénicaud L

机构信息

Laboratoire de Physiopathologie de la Nutrition, CNRS, Paris, France.

出版信息

Brain Res Mol Brain Res. 1996 May;38(1):45-53. doi: 10.1016/0169-328x(95)00306-d.

DOI:10.1016/0169-328x(95)00306-d

PMID:8737666

Abstract

Whether or not glucose utilization in the brain is insulin-dependent is still a controversial issue. We looked for the presence of the insulin-sensitive glucose transporter (GLUT4) in rat brain and obtained the following results: (1) poly(A) RNAs from the hypothalamus and anterior medulla oblongata hybridize with a cDNA probe for GLUT4; (2) reverse transcription-polymerase chain reaction (RT-PCR) on RNA from various brain nuclei detects GLUT4 transcripts; (3) immunocytochemistry, using a polyclonal antibody to GLUT4; reveals a specific immunostaining pattern, whereas both electronic microscopy and double immunofluorescence staining, using a neurofilament protein marker, indicate a neuronal localization. These results are discussed in terms of a putative neuromodulator role of insulin, via glucose utilization, in brain areas involved in the regulation of fuel metabolism.

摘要

大脑中葡萄糖的利用是否依赖胰岛素仍是一个有争议的问题。我们研究了大鼠脑中胰岛素敏感性葡萄糖转运体（GLUT4）的存在情况，并得到了以下结果：（1）来自下丘脑和延髓前部的多聚腺苷酸RNA与GLUT4的cDNA探针杂交；（2）对来自不同脑核的RNA进行逆转录聚合酶链反应（RT-PCR）检测到GLUT4转录本；（3）使用针对GLUT4的多克隆抗体进行免疫细胞化学分析，显示出特定的免疫染色模式，而电子显微镜检查以及使用神经丝蛋白标记物的双重免疫荧光染色均表明其定位于神经元。本文根据胰岛素可能通过葡萄糖利用在参与调节能量代谢的脑区中发挥神经调节作用这一观点对这些结果进行了讨论。