Lawson A, England M A
Department of Anatomy, University of Ghana Medical School, Accra, Ghana.
Anat Embryol (Berl). 1996 Jun;193(6):601-10. doi: 10.1007/BF00187932.
The role of increased cerebrospinal fluid pressure and morphogenetic brain expansion on midbrain wound healing was studied in chick embryos at stages 16-22. The embryos were divided into six groups as follows: group I (stages 16/17), group II (stages 18/19), group III (stages 20-22), group IV (stages 18/19), group V (stages 20-22) and group VI (stages 18/19). The mid-brains of embryos of groups I-III were wounded and the embryos re-incubated for varying periods up to 24 h. The neuroepithelial wounds of all group-I embryos healed completely within 24 h. However, complete healing was observed in only 25% of wounds in group II and 11.4% in group III by 24 h. To reduce cerebrospinal fluid pressure and thus slow down brain expansion, longitudinal wounds (about 0.8 mm long) were made in the hindbrain roof plate of group-IV and group-V embryos, and puncture wounds (0.1 mm in diameter) also in the hindbrain roof plate of group-VI embryos. This allowed cerebrospinal fluid to escape prior to wounding the midbrain. There was a significant increase in the proportion of group-IV and group-V embryos with completely healed midbrain neuroepithelial wounds (77.3% and 28.6% respectively). However, a comparison between groups II and VI embryos yielded no statistically significant difference in healing. Thus, increasing cerebrospinal fluid pressure and brain expansion adversely affect midbrain neuroepithelial wound healing.
在16 - 22期的鸡胚中研究了脑脊液压力升高和脑形态发生性扩张对中脑伤口愈合的作用。将胚胎分为以下六组：第一组（16/17期）、第二组（18/19期）、第三组（20 - 22期）、第四组（18/19期）、第五组（20 - 22期）和第六组（18/19期）。对第一至三组胚胎的中脑进行创伤，然后将胚胎重新孵化不同时长直至24小时。所有第一组胚胎的神经上皮伤口在24小时内完全愈合。然而，到24小时时，第二组中只有25%的伤口完全愈合，第三组中只有11.4%的伤口完全愈合。为了降低脑脊液压力从而减缓脑扩张，在第四组和第五组胚胎的后脑顶板上制造纵向伤口（约0.8毫米长），在第六组胚胎的后脑顶板上制造穿刺伤口（直径0.1毫米）。这使得脑脊液在中脑创伤之前得以排出。第四组和第五组胚胎中脑神经上皮伤口完全愈合的比例显著增加（分别为77.3%和28.6%）。然而，第二组和第六组胚胎之间在愈合方面没有统计学上的显著差异。因此，脑脊液压力升高和脑扩张对中脑神经上皮伤口愈合产生不利影响。
