Schwartz D, Perry R, Dombroski D M, Merrick J M, Goldhar J
Microbiology Laboratory, Sourasky (Ichilov) Medical Center, Tel-Aviv, Israel.
Zentralbl Bakteriol. 1996 Apr;283(4):485-91. doi: 10.1016/s0934-8840(96)80125-3.
The invasive ability of C. jejuni/coli strains isolated from children with diarrhea was studied using an in vitro HEp-2 cell invasion assay. The ratio between the number of intracellular bacteria and the number of bacteria in the inoculum was determined (invasion index). It was found that under anaerobic conditions, there was a significant decrease in the invasion index as compared to standard conditions (5% CO2). Of 11 strains tested, seven were determined as invasive on the basis of invasion indexes within the range of 0.0002-0.01. In a previous study [D. Schwartz et al., Zbl. Bakt. 280, 338-347 (1994)], it was found, that most of the C. jejuni/coli isolates tested produced an outer membrane protein when grown under conditions of iron depletion (IRP). The IRP were detected in eight of the nine strains tested in the present study (five invasive and three non-invasive strains). In one non-invasive strain, IRP was not detected. When kept under conditions of iron depletion, one of the invasive strains exhibited a significant increase in invasive capacity. The results suggest that iron depletion seems to stimulate the invasion capacity of C. jejuni/coli in vitro.
采用体外HEp - 2细胞侵袭试验研究了从腹泻儿童中分离出的空肠弯曲菌/结肠弯曲菌菌株的侵袭能力。测定了细胞内细菌数量与接种物中细菌数量的比值（侵袭指数）。结果发现，与标准条件（5%二氧化碳）相比，在厌氧条件下侵袭指数显著降低。在测试的11株菌株中，有7株根据侵袭指数在0.0002 - 0.01范围内被确定为具有侵袭性。在之前的一项研究[D. Schwartz等人，《中央细菌学杂志》280, 338 - 347 (1994)]中发现，大多数测试的空肠弯曲菌/结肠弯曲菌分离株在缺铁条件（IRP）下生长时会产生一种外膜蛋白。在本研究测试的9株菌株中的8株（5株侵袭性菌株和3株非侵袭性菌株）中检测到了IRP。在一株非侵袭性菌株中未检测到IRP。当在缺铁条件下保存时，其中一株侵袭性菌株的侵袭能力显著增加。结果表明，缺铁似乎在体外刺激了空肠弯曲菌/结肠弯曲菌的侵袭能力。
