Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland.
Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland.
Microb Pathog. 2018 Feb;115:257-263. doi: 10.1016/j.micpath.2017.12.057. Epub 2017 Dec 20.
The aim of the study was to determine the prevalence of virulence genes responsible for the adhesion (flaA, cadF and racR) and invasion (virB11, iam and pldA) in Campylobacter isolates from cattle and swine and determine their adherence and invasion abilities. The studies conducted revealed high prevalence rate of adherence and invasion associated genes irrespective of the isolates origin. All Campylobacter strains of swine and cattle origin adhered to HeLa cells at mean level 0.1099% ± SD 0.1341% and 0.0845% ± SD 0.1304% of starting viable inoculum, respectively. However swine isolates exhibited higher invasion abilities (0.0012% ± SD 0.0011%) compared to bovine isolates (0.00038% ± SD 0.00055%). The results obtained revealed significantly positive correlation between invasion and adherence abilities of swine origin isolates (R = 0.4867 in regard to C. jejuni and R = 0.4507 in regard to C. coli) and bovine origin isolates (R = 0.726 in regard to C. jejuni). Bacterial virulence is multifactorial and it is affected by the expression of virulence genes. Moreover the presence of virulence genes determines the ability of Campylobacter isolates to adhere and invade the cells.
本研究旨在确定与粘附（flaA、cadF 和 racR）和侵袭（virB11、iam 和 pldA）相关的毒力基因在牛和猪源空肠弯曲菌分离株中的流行率，并确定其粘附和侵袭能力。研究结果表明，无论分离株的来源如何，粘附和侵袭相关基因的流行率都很高。所有来自猪和牛的空肠弯曲菌菌株分别以起始活菌接种量的 0.1099%±0.1341%和 0.0845%±0.1304%的平均水平粘附至 HeLa 细胞。然而，猪源分离株的侵袭能力更高（0.0012%±0.0011%），而牛源分离株的侵袭能力较低（0.00038%±0.00055%）。结果表明，猪源分离株的侵袭和粘附能力之间存在显著的正相关（在空肠弯曲菌方面，R=0.4867；在大肠弯曲菌方面，R=0.4507），而牛源分离株的侵袭和粘附能力之间也存在显著的正相关（在空肠弯曲菌方面，R=0.726）。细菌的毒力是多因素的，受到毒力基因的表达影响。此外，毒力基因的存在决定了空肠弯曲菌分离株粘附和侵袭细胞的能力。
