通过固相萃取和高效液相色谱法测定动物和人体血浆中的一种HIV蛋白酶抑制剂（DMP 450）。
Determination of a HIV protease inhibitor (DMP 450) in animal and human plasma by solid-phase extraction and high-performance liquid chromatography.
作者信息
Grubb M F, Robinson C A, White S J, Krauthauser C L, Shum L
机构信息
Drug Metabolism and Pharmacokinetics Section, DuPont Merck Pharmaceutical Company, Stine-Haskell Research Center, Newark, DE 19714, USA.
出版信息
J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):303-8. doi: 10.1016/0378-4347(95)00478-5.
Extraction of DMP 450 from plasma was performed with C2 solid-phase extraction columns, using 0.1 M ammonium acetate in 90% methanol to elute DMP 450. The extraction recovery over the range of 10 to 10 000 ng/ml averaged 81.0, 96.2, 77.4, 95.2 and 68.0% from rat, dog, monkey, chimpanzee (25-10 000 ng/ml) and human plasma, respectively. HPLC analysis was carried out with a C18 column and a mobile phase of acetonitrile, methanol and 30 mM potassium phosphate (pH 3), the composition dependent on the type of plasma being analyzed, and monitored at a wavelength of 229 nm. Intra-day and inter-day coefficients of variation were less than 9.9 and 12.9%, respectively. Absolute differences were less than 11.5%.
使用C2固相萃取柱从血浆中提取DMP 450，用含0.1 M醋酸铵的90%甲醇洗脱DMP 450。在10至10000 ng/ml范围内，从大鼠、狗、猴、黑猩猩（25 - 10000 ng/ml）和人血浆中提取DMP 450的回收率平均分别为81.0%、96.2%、77.4%、95.2%和68.0%。采用C18柱进行HPLC分析，流动相为乙腈、甲醇和30 mM磷酸钾（pH 3），其组成取决于所分析血浆的类型，并在229 nm波长处进行监测。日内和日间变异系数分别小于9.9%和12.9%。绝对差异小于11.5%。
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