Vletter A A, Olieman W, Burm A G, Groen K, van Kleef J W
University Hospital Leiden, Department of Anaesthesiology, The Netherlands.
J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):369-72. doi: 10.1016/0378-4347(95)00568-4.
A method enabling quantification of R-(-)- and S-(+)-mepivacaine in human plasma in the low nanogram per milliliter range is described. The procedure involves extraction from plasma with diethyl ether, centrifugation, back-extraction into an acidified aqueous solution, washing with a mixture of pentane and isoamylalcohol, alkalinisation, followed by extraction with a mixture of n-pentane and isoamylalcohol. After evaporation of the organic phase, the residue is redissolved in the mobile phase used for the HPLC analysis, which consists of a 6.8:93.2 (v/v) isopropanol-sodium hydrogenphosphate buffer solution with the pH adjusted to 6.8 using phosphoric acid. The HPLC method has been described previously. Separation of the enantiomers is achieved with an alpha 1-AGP column and the UV detection wavelength is 210 nm. The minimal detectable concentration is ca. 3 ng/ml and the lower limit of quantification is 5 ng/ml for each enantiomer. For both enantiomers r is > 0.9995 over the plasma enantiomeric concentration range of 10.5-1053 ng/ml.
描述了一种能够在低纳克每毫升范围内对人血浆中R-(-)-和S-(+)-甲哌卡因进行定量的方法。该过程包括用乙醚从血浆中萃取、离心、反萃取到酸化水溶液中、用戊烷和异戊醇的混合物洗涤、碱化，然后用正戊烷和异戊醇的混合物萃取。有机相蒸发后，残留物重新溶解在用于高效液相色谱分析的流动相中，该流动相由6.8:93.2（v/v）的异丙醇-磷酸氢二钠缓冲溶液组成，用磷酸将pH值调至6.8。高效液相色谱法先前已有描述。对映体的分离通过α1-AGP柱实现，紫外检测波长为210nm。最低检测浓度约为3ng/ml，每种对映体的定量下限为5ng/ml。在10.5-1053ng/ml的血浆对映体浓度范围内，两种对映体的r均>0.9995。
