Chiba K, Ishikawa H, Rahman M E, Endo A
Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, Japan.
Reprod Toxicol. 1996 May-Jun;10(3):215-9. doi: 10.1016/0890-6238(96)00024-x.
To induce hip joint anomalies and assess their relationship with hindlimb anomalies, pregnant mice (Jcl;ICR) were injected intraperitoneally with a single dose of 5.0 or 7.5 mg/kg of cytosine arabinoside (Ara-C) on dg 8, 9.5, or 11. On the 24th postnatal day, surviving offspring were stained by alizarin red S and anomalies were observed. Hip joint anomalies were observed only in one group exposed to 7.5 mg/kg on dg 9.5; the incidence of the hip joint anomalies in this group was about 30%. The types of hip anomalies observed were femoral shaft dysplasia, pseudoarthrosis of the femur, femoral head dysplasia, acetabular dysplasia, fusion between the femoral head and acetabulum, and pseudoarthrosis of the coxal bone. All of these anomalies were associated with preaxial hyperplasia of the hind paws and lower leg anomalies. Strangely, while newborns with no hip joint anomalies had a fairly high rate of oligodactyly, no newborn with hip joint anomalies had oligodactyly.
为诱导髋关节异常并评估其与后肢异常的关系，在妊娠第8、9.5或11天，给怀孕小鼠（Jcl;ICR）腹腔注射单剂量5.0或7.5mg/kg的阿糖胞苷（Ara-C）。在出生后第24天，对存活的后代用茜素红S染色并观察异常情况。仅在妊娠第9.5天接受7.5mg/kg剂量的一组中观察到髋关节异常；该组髋关节异常的发生率约为30%。观察到的髋关节异常类型包括股骨干发育不良、股骨假关节、股骨头发育不良、髋臼发育不良、股骨头与髋臼融合以及髋骨假关节。所有这些异常均与后爪的轴前增生和小腿异常有关。奇怪的是，虽然无髋关节异常的新生儿多指畸形发生率相当高，但有髋关节异常的新生儿均无多指畸形。
