睾丸酮假单胞菌中的邻苯二甲酸酯代谢：突变菌株积累4,5-二羟基邻苯二甲酸
Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain.
作者信息
Nakazawa T, Hayashi E
出版信息
J Bacteriol. 1977 Jul;131(1):42-8. doi: 10.1128/jb.131.1.42-48.1977.
Abstract
A mutant strain of Pseudomonas testosteroni blocked in phthalate catabolism converted phthalate into 4,5-dihydroxyphthalate. The latter compound was isolated, and its physical properties were determined. A stoichiometric conversion of the compound to protocatechuate was demonstrated spectrophotometrically with crude extracts of a protocatechuate 4,5-dioxygenase-deficient mutant. Therefore, phthalate is metabolized through 4,5-dihydroxyphthalate and protocatechuate, which is further degraded by protocatechuate 4,5-dioxygenase in P. testosteroni. By using several mutants blocked in phthalate catabolism, 4,5-dihydroxyphthalate decarboxylase was shown to be induced by phthalate. A simple spectrophotometric assay for the enzyme is also reported.
摘要
一株在邻苯二甲酸酯分解代谢中受阻的睾丸酮假单胞菌突变株将邻苯二甲酸酯转化为4,5 - 二羟基邻苯二甲酸酯。分离出了后一种化合物，并测定了其物理性质。用原儿茶酸4,5 - 双加氧酶缺陷型突变株的粗提取物通过分光光度法证明了该化合物向原儿茶酸的化学计量转化。因此，邻苯二甲酸酯通过4,5 - 二羟基邻苯二甲酸酯和原儿茶酸进行代谢，原儿茶酸在睾丸酮假单胞菌中进一步被原儿茶酸4,5 - 双加氧酶降解。通过使用几种在邻苯二甲酸酯分解代谢中受阻的突变株，表明4,5 - 二羟基邻苯二甲酸酯脱羧酶是由邻苯二甲酸酯诱导产生的。还报道了一种针对该酶的简单分光光度测定法。
相似文献
1
Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain.睾丸酮假单胞菌中的邻苯二甲酸酯代谢：突变菌株积累4,5-二羟基邻苯二甲酸
J Bacteriol. 1977 Jul;131(1):42-8. doi: 10.1128/jb.131.1.42-48.1977.
2
Phthalate and 4-hydroxyphthalate metabolism in Pseudomonas testosteroni: purification and properties of 4,5-dihydroxyphthalate decarboxylase.睾丸酮假单胞菌中邻苯二甲酸酯和4-羟基邻苯二甲酸酯的代谢：4,5-二羟基邻苯二甲酸脱羧酶的纯化及特性
Appl Environ Microbiol. 1978 Aug;36(2):264-9. doi: 10.1128/aem.36.2.264-269.1978.
3
Phthalate metabolism in Pseudomonas fluorescens PHK: purification and properties of 4,5-dihydroxyphthalate decarboxylase.荧光假单胞菌PHK中的邻苯二甲酸酯代谢：4,5-二羟基邻苯二甲酸脱羧酶的纯化及特性
Appl Environ Microbiol. 1985 Feb;49(2):374-6. doi: 10.1128/aem.49.2.374-376.1985.
4
Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B.微球菌属菌株12B对邻苯二甲酸二丁酯和邻苯二甲酸酯的代谢
J Bacteriol. 1982 Jul;151(1):48-57. doi: 10.1128/jb.151.1.48-57.1982.
5
Biodegradation of the phthalates and their esters by bacteria.细菌对邻苯二甲酸盐及其酯类的生物降解作用。
Environ Health Perspect. 1976 Dec;18:159-66. doi: 10.1289/ehp.7618159.
6
2,3-Dihydroxybenzoate meta-Cleavage Pathway is Involved in o-Phthalate Utilization in Pseudomonas sp. strain PTH10.邻苯二甲酸在假单胞菌 PTH10 中的利用涉及 2,3-二羟基苯甲酸间位裂解途径。
Sci Rep. 2019 Feb 4;9(1):1253. doi: 10.1038/s41598-018-38077-2.
7
Molecular and biochemical analysis of phthalate and terephthalate degradation by Rhodococcus sp. strain DK17.红球菌属DK17菌株对邻苯二甲酸酯和对苯二甲酸酯降解的分子与生化分析
FEMS Microbiol Lett. 2005 Nov 15;252(2):207-13. doi: 10.1016/j.femsle.2005.08.045. Epub 2005 Sep 12.
8
Plasmid-encoded phthalate catabolic pathway in Arthrobacter keyseri 12B.凯氏节杆菌12B中质粒编码的邻苯二甲酸分解代谢途径。
J Bacteriol. 2001 Jun;183(12):3689-703. doi: 10.1128/JB.183.12.3689-3703.2001.
9
Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17.红球菌属菌株DK17对邻苯二甲酸盐进行最大代谢所需的重复操纵子。
Biochem Biophys Res Commun. 2007 Jun 8;357(3):766-71. doi: 10.1016/j.bbrc.2007.04.009. Epub 2007 Apr 10.
10
Molecular characterization of the genes pcaG and pcaH, encoding protocatechuate 3,4-dioxygenase, which are essential for vanillin catabolism in Pseudomonas sp. strain HR199.编码原儿茶酸3,4-双加氧酶的基因pcaG和pcaH的分子特征，该酶对假单胞菌属菌株HR199中香草醛的分解代谢至关重要。
Appl Environ Microbiol. 1999 Mar;65(3):951-60. doi: 10.1128/AEM.65.3.951-960.1999.
引用本文的文献
1
2,3-Dihydroxybenzoate meta-Cleavage Pathway is Involved in o-Phthalate Utilization in Pseudomonas sp. strain PTH10.邻苯二甲酸在假单胞菌 PTH10 中的利用涉及 2,3-二羟基苯甲酸间位裂解途径。
Sci Rep. 2019 Feb 4;9(1):1253. doi: 10.1038/s41598-018-38077-2.
2
Involvement of a novel ABC transporter and monoalkyl phthalate ester hydrolase in phthalate ester catabolism by Rhodococcus jostii RHA1.新型 ABC 转运蛋白和单烷烃邻苯二甲酸酯水解酶参与罗得西亚棒杆菌 RHA1 对邻苯二甲酸酯的代谢。
Appl Environ Microbiol. 2010 Mar;76(5):1516-23. doi: 10.1128/AEM.02621-09. Epub 2009 Dec 28.
3
Transcriptomic analysis reveals a bifurcated terephthalate degradation pathway in Rhodococcus sp. strain RHA1.转录组分析揭示了红球菌属菌株RHA1中对苯二甲酸的分叉降解途径。
J Bacteriol. 2007 Mar;189(5):1641-7. doi: 10.1128/JB.01322-06. Epub 2006 Dec 1.
4
Bacterial Decarboxylation of o-Phthalic Acids.邻苯二甲酸的细菌脱羧作用。
Appl Environ Microbiol. 1983 Dec;46(6):1276-81. doi: 10.1128/aem.46.6.1276-1281.1983.
5
Plasmid-encoded phthalate catabolic pathway in Arthrobacter keyseri 12B.凯氏节杆菌12B中质粒编码的邻苯二甲酸分解代谢途径。
J Bacteriol. 2001 Jun;183(12):3689-703. doi: 10.1128/JB.183.12.3689-3703.2001.
6
Novel organization of the genes for phthalate degradation from Burkholderia cepacia DBO1.洋葱伯克霍尔德菌DBO1中邻苯二甲酸酯降解基因的新组织形式。
J Bacteriol. 1998 Dec;180(24):6529-37. doi: 10.1128/JB.180.24.6529-6537.1998.
7
Degradation of bis(2-ethylhexyl) phthalate constituents under methanogenic conditions.邻苯二甲酸二（2-乙基己基）酯成分在产甲烷条件下的降解
Biodegradation. 1996;7(6):501-6. doi: 10.1007/BF00115296.
8
2-Naphthoate catabolic pathway in Burkholderia strain JT 1500.伯克霍尔德氏菌JT 1500菌株中的2-萘甲酸盐分解代谢途径。
J Bacteriol. 1997 Jan;179(1):115-21. doi: 10.1128/jb.179.1.115-121.1997.
9
Utilization of phthalate esters by micrococci.微球菌对邻苯二甲酸酯的利用
Arch Microbiol. 1982 Aug;132(2):185-8. doi: 10.1007/BF00508728.
10
Metabolism of dibutylphthalate and phthalate by Micrococcus sp. strain 12B.微球菌属菌株12B对邻苯二甲酸二丁酯和邻苯二甲酸酯的代谢
J Bacteriol. 1982 Jul;151(1):48-57. doi: 10.1128/jb.151.1.48-57.1982.
本文引用的文献
1
Oxidative metabolism of phthalic acid by soil pseudomonads.土壤假单胞菌对邻苯二甲酸的氧化代谢
Biochem J. 1960 Aug;76(2):310-8. doi: 10.1042/bj0760310.
2
New pathways in the oxidative metabolism of aromatic compounds by microorganisms.微生物对芳香族化合物氧化代谢的新途径。
Nature. 1960 Nov 12;188:560-6. doi: 10.1038/188560a0.
3
The aerobic pseudomonads: a taxonomic study.需氧假单胞菌属：一项分类学研究。
J Gen Microbiol. 1966 May;43(2):159-271. doi: 10.1099/00221287-43-2-159.
4
Deoxyribonucleic acid base composition in the genus Pseudomonas.假单胞菌属中的脱氧核糖核酸碱基组成。
J Gen Microbiol. 1966 May;43(2):273-92. doi: 10.1099/00221287-43-2-273.
5
Toxicology of a series of phthalate esters.一系列邻苯二甲酸酯的毒理学
J Pharm Sci. 1966 Feb;55(2):158-62. doi: 10.1002/jps.2600550206.
6
The metabolism of aromatic acids by Pseudomonas testosteroni and P. acidovorans.睾丸酮假单胞菌和食酸假单胞菌对芳香酸的代谢
Arch Mikrobiol. 1967;59(1):302-14. doi: 10.1007/BF00406344.
7
Purification and some properties of protocatechuate 4,5-dioxygenase.原儿茶酸4,5-双加氧酶的纯化及某些性质
Biochim Biophys Acta. 1970 Nov 11;220(2):224-38. doi: 10.1016/0005-2744(70)90008-2.
8
Phthalate esters as environmental contaminants.邻苯二甲酸酯作为环境污染物。
Nature. 1972 Aug 18;238(5364):411-3. doi: 10.1038/238411a0.
9
Metabolism of benzoic acid by bacteria: 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid is an intermediate in the formation of catechol.细菌对苯甲酸的代谢：3,5-环己二烯-1,2-二醇-1-羧酸是儿茶酚形成过程中的一种中间体。
J Bacteriol. 1971 Oct;108(1):89-94. doi: 10.1128/jb.108.1.89-94.1971.
10
Degradation of protocatechuate in Pseudomonas testosteroni by a pathway involving oxidation of the product of meta-fission.睾丸酮假单胞菌中通过涉及间位裂解产物氧化的途径降解原儿茶酸。
J Bacteriol. 1973 Jan;113(1):521-3. doi: 10.1128/jb.113.1.521-523.1973.
Zotero 插件