Hattorl A, Tsujimoto M, Hayashi K, Kohno M
Department of Biology, Gifu Pharmaceutical University, Japan.
Biochem Mol Biol Int. 1996 May;38(6):1095-101.
A possible interaction between tumor necrosis factor (TNF) and transforming growth factor-beta (TGF-beta) superfamily cytokines in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. Although TGF-beta 1 and Activin A stimulated NGF production in Swiss 3T3 cells, they antagonized the stimulatory effect of TNF on fibroblast NGF production when used in combination. On the contrary, TNF's stimulatory activity on fibroblast NGF production was markedly synergized by bone morphogenetic protein-2 (BMP-2); BMP-2 by itself did not stimulate NGF production in the cells. These findings suggest that BMP-2, in concert with TNF, plays an essential role in regulating the regeneration of peripheral nerves following injury with bone fracture through an indirect mechanism by which it stimulates NGF production in fibroblasts.
研究了肿瘤坏死因子（TNF）与转化生长因子-β（TGF-β）超家族细胞因子在刺激瑞士3T3细胞产生神经生长因子（NGF）过程中可能存在的相互作用。尽管TGF-β1和激活素A可刺激瑞士3T3细胞产生NGF，但它们联合使用时会拮抗TNF对成纤维细胞NGF产生的刺激作用。相反，骨形态发生蛋白-2（BMP-2）可显著增强TNF对成纤维细胞NGF产生的刺激活性；BMP-2本身并不会刺激细胞产生NGF。这些发现表明，BMP-2与TNF协同作用，通过刺激成纤维细胞产生NGF这一间接机制，在调节骨折损伤后周围神经的再生中发挥着重要作用。
