Hatakeyama S, Ohara-Nemoto Y, Kyakumoto S, Satoh M
Department of Oral Pathology, School of Dentistry, Iwate Medical University, Morioka, Japan.
Biochem Mol Biol Int. 1996 May;38(6):1235-43.
Expression of bone morphogenetic protein (BMP)-2 mRNA was stimulated by retinoic acid in human adenocarcinoma cell line, HSG-S8, in a dose-dependent manner. Northern blot analysis demonstrated that retinoic acid most strongly increased the level of BMP-2 mRNA 6 h after the treatment and the stimulatory effect was maintained at 48 h. The mature peptides of 16 and 18 kDa molecular masses of BMP-2 were also increased in the conditioned medium by the treatment of retinoic acid on western blotting. The proliferation of HSG-S8 cells was inhibited by retinoic acid, however, retinoic acid did not cause morphological change showing cellular differentiation. 1 alpha, 25(OH)2D3, like retinoic acid, clearly increased the mRNA level of BMP-2, whereas dibuthryl cyclic AMP remarkably diminished it, and bromodeoxyuridine had no effect on the expression of BMP-2 mRNA.
在人腺癌细胞系HSG-S8中，视黄酸以剂量依赖的方式刺激骨形态发生蛋白（BMP）-2 mRNA的表达。Northern印迹分析表明，视黄酸在处理后6小时最强烈地增加了BMP-2 mRNA的水平，并且刺激作用在48小时时得以维持。通过视黄酸处理，条件培养基中16 kDa和18 kDa分子量的成熟肽在蛋白质印迹上也有所增加。视黄酸抑制了HSG-S8细胞的增殖，然而，视黄酸并未引起显示细胞分化的形态学变化。1α，25（OH）2D3与视黄酸一样，明显增加了BMP-2的mRNA水平，而二丁酰环磷腺苷明显降低了该水平，并且溴脱氧尿苷对BMP-2 mRNA的表达没有影响。
