Pajer Z, Cör A
Institute of Histology and Embryology, Faculty of Medicine, Ljubljana, Slovenia.
Pflugers Arch. 1996;431(6 Suppl 2):R209-10. doi: 10.1007/BF02346342.
FRTL-5 cells grow as a monolayer culture in vitro and they must be detached from the solid surface by trypsin before reseeding. The aim of this study was to examine by light microscopy and by the astereological method the changes that occur in FRTL-5 cells one, two, three or four days after trypsinisation. The most pronounced qualitative and quantitative changes were seen from the first to the second day after trypsinisation, as the area of the cells increased by 119%, their nuclei by 71% and the cytoplasm by 150%, while the nuclear-cytoplasmic ratio decreased by 42%. Significant increases in the average area of the cells, their average nuclear diameter and perimeter were seen also on the third and fourth days, while the average nuclear-cytoplasmic ratio decreased significantly on the first three days after trypsinisation and then remained constant.
FRTL-5细胞在体外以单层培养方式生长，在重新接种前必须用胰蛋白酶将它们从固体表面分离下来。本研究的目的是通过光学显微镜和体视学方法检查胰蛋白酶消化后1天、2天、3天或4天FRTL-5细胞中发生的变化。从胰蛋白酶消化后的第一天到第二天观察到最明显的定性和定量变化，细胞面积增加了119%，细胞核增加了71%，细胞质增加了150%，而核质比下降了42%。在第三天和第四天，细胞的平均面积、平均核直径和周长也显著增加，而在胰蛋白酶消化后的前三天，平均核质比显著下降，然后保持不变。
