Merali F S, Arck P C, Beaman K, Clark D A
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Am J Reprod Immunol. 1996 Apr;35(4):342-7. doi: 10.1111/j.1600-0897.1996.tb00491.x.
Transforming growth factor (TGF)-beta 2-related-decidual suppressor factor (DSF) and TJ6 protein are both immunosuppressive molecules present in murine and human pregnancy. Treatment of mice with either anti-TJ6 or anti-TGF-beta 2 neutralizing antibodies results in increased fetal loss. Western blots of supernatants from pregnant mouse decidua probed with anti-TJ6 (soluble form) showed a doublet at a similar molecular size as when the blot was probed with anti-TGF-beta 2 antibody. The problem is to determine whether TJ6 and DSF are the same protein. In order to determine if TJ6 and DSF are the same or different proteins, we used affinity column purified TGF-beta 2-DSF and stained Western blots with anti-TJ6. The TGF-beta 2-monoclonal antibody affinity column-purified DSF that stained with anti TGF-beta 2 was not reactive with anti-TJ6 antibody. TJ6 has only a 30% gene sequence homology and a 13% amino acid homology to TGF-beta 2. TJ6 and TGF-beta 2-related DSF appear to be different immunosuppressive proteins in decidua.
转化生长因子（TGF）-β2相关蜕膜抑制因子（DSF）和TJ6蛋白都是存在于小鼠和人类孕期的免疫抑制分子。用抗TJ6或抗TGF-β2中和抗体处理小鼠会导致胎儿丢失增加。用抗TJ6（可溶性形式）探测怀孕小鼠蜕膜上清液的蛋白质免疫印迹显示，与用抗TGF-β2抗体探测印迹时相比，在相似的分子大小处出现了一条双条带。问题在于确定TJ6和DSF是否为同一蛋白。为了确定TJ6和DSF是相同还是不同的蛋白，我们使用亲和柱纯化的TGF-β2-DSF并用抗TJ6对蛋白质免疫印迹进行染色。用抗TGF-β2染色的TGF-β2单克隆抗体亲和柱纯化的DSF与抗TJ6抗体无反应。TJ6与TGF-β2的基因序列同源性仅为30%，氨基酸同源性为13%。TJ6和TGF-β2相关的DSF似乎是蜕膜中不同的免疫抑制蛋白。
