Kordek R, Biernat W, Debiec-Rychter M, Alwasiak J, Liberski P P
Department of Oncology, Medical University of Lodz, Poland.
Pathol Res Pract. 1996 Mar;192(3):205-9. doi: 10.1016/S0344-0338(96)80222-6.
Mutations of the p53 gene are one of the most frequent genomic alterations of human tumours of astrocytic lineage. Because the physiological role of this gene is a suppression of cellular proliferation and growth, the overexpression of p53-protein may correlate with the expression of PCNA or Ki-67, established markers of cell proliferation. Paraffin-embedded surgical specimens from 60 human astrocytomas (9 pilocytic tumours, 12 WHO grade II, 9 anaplastic astrocytomas [WHO grade III] and 30 glioblastomas [WHO grade IV]) were stained with anti-PCNA (PC10), anti-p53(DO-7) and anti-Ki-67 antibodies (DAKO). Approximately 40% of all the cases were p53-protein immunopositive (53.3% glioblastomas, 33.3% anaplastic, 41.7% low grade astrocytomas but no pilocytic tumor). Statistical analysis did not reveal statistically significant correlation between p53-immunopositivity and PCNA or Ki-67 labeling indices. The Ki-67- and PCNA LI-s were statistically correlated, and the former better discriminated groups of different grades of malignancy.
p53基因的突变是星形细胞谱系人类肿瘤中最常见的基因组改变之一。由于该基因的生理作用是抑制细胞增殖和生长，p53蛋白的过表达可能与细胞增殖的既定标志物PCNA或Ki-67的表达相关。用抗PCNA（PC10）、抗p53（DO-7）和抗Ki-67抗体（DAKO）对60例人类星形细胞瘤（9例毛细胞型肿瘤、12例WHO二级、9例间变性星形细胞瘤[WHO三级]和30例胶质母细胞瘤[WHO四级]）的石蜡包埋手术标本进行染色。所有病例中约40%为p53蛋白免疫阳性（胶质母细胞瘤为53.3%，间变性为33.3%，低级别星形细胞瘤为41.7%，但毛细胞型肿瘤无阳性）。统计分析未显示p53免疫阳性与PCNA或Ki-67标记指数之间存在统计学显著相关性。Ki-67和PCNA标记指数具有统计学相关性，前者能更好地区分不同恶性程度的组。
