Kordek R, Biernat W, Alwasiak J, Liberski P P
Department of Pathology, Medical University of Lódź, Poland.
Acta Neurochir (Wien). 1996;138(5):509-12; discussion 513. doi: 10.1007/BF01411168.
To compare the Ki-67 and Proliferating Cell Nuclear Antigen (PCNA), markers of cell proliferation, paraffin embedded surgical specimens from 56 human astrocytic tumours (8 pilocytic tumours, grade I; 9 low (II) grade and 9 anaplastic (grade III) astrocytomas and 30 glioblastomas, grade IV) were immunolabelled with the anti-PCNA (PC 10, DAKO) and anti-Ki-67 (DAKO) antibodies. For the latter immunostaining the microwave oven processing was performed. The Ki-67 and PCNA labelling indices (LIs) were statistically compared. For the majority of cases, PCNA LI was higher than that obtained with anti-Ki-67 antibody and the intensity of staining for PCNA was more variable. The statistically significant difference of the percentage of PCNA LIs was found only between low grade (I and II) and high grade (III and IV) tumours, while Ki-67 LIs discriminates each group of glial tumours; thus this latter marker is more sensitive and specific.
为比较细胞增殖标志物Ki-67和增殖细胞核抗原（PCNA），对56例人类星形细胞瘤（8例一级毛细胞型肿瘤、9例二级低级别和9例间变性（三级）星形细胞瘤以及30例四级胶质母细胞瘤）的石蜡包埋手术标本，用抗PCNA（PC 10，丹麦戴科公司）和抗Ki-67（丹麦戴科公司）抗体进行免疫标记。对于后者的免疫染色采用微波炉处理。对Ki-67和PCNA标记指数（LIs）进行统计学比较。在大多数病例中，PCNA LI高于抗Ki-67抗体所获得的结果，且PCNA的染色强度变化更大。仅在低级别（一级和二级）与高级别（三级和四级）肿瘤之间发现PCNA LIs百分比存在统计学显著差异，而Ki-67 LIs能区分每组胶质肿瘤；因此后一种标志物更敏感且更具特异性。
